| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1598SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56326SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25162SS | |||
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0008.jpg "Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - ICC staining of Caspase-9 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primar](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0005.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded human tonsil tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0004.jpg)
![Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Western-Blot-NB100-1598-img0013.jpg "Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1598-img0010.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0012.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0011.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-15053-img0015.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: OCT4 Antibody [NBP2-15053] - Human embryonic stem cells, staining with OCT3/4 or rabbit IgG (black).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NBP2-15053-img0001.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0007.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP2-15053] - Analysis of hESCs on CF1 feeders, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15053-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody [NBP2-15053] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15053_rabbit-polyclonal-oct4-antibody-1610202419414555.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody [NBP2-19459] - Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0019.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0014.jpg)
![Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Western-Blot-NBP2-19459-img0023.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0008.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19459-img0018.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251842291.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-immunocytochemistry-immunofluorescence-261120251825260.jpg)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract5% SDS-PAGEN-Cadherin antibody [N1N3] (NBP2-19459) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19459_rabbit-polyclonal-n-cadherin-antibody-western-blot-261120251829017.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Western-Blot-NBP2-29420-img0007.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Immunocytochemistry-Immunofluorescence-NBP2-29420-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0001.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Electropherogram image of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0004.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0010.jpg "Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.")
![Western Blot: Nrf1 Antibody [NBP2-19586] - IP samples: 293T whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NRF1 protein by 4 ug NRF1 antibody. 10 % SDS-PAGE The immunoprecipitated NRF1 protein was detected by NRF1 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP2-19586-img0003.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded human lung adenocarcinoma. NRF1 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0006.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse muscle. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP2-19586] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF1 stained by NRF1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10μm.](http://images.novusbio.com/fullsize/antibody/nbp2-19586_rabbit-polyclonal-nrf1-antibody-immunocytochemistry-immunofluorescence-582024111857..jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Nrf1 antibody detects Nrf1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Nrf1 stained by Nrf1 antibody (NBP2-19586) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19586_rabbit-polyclonal-nrf1-antibody-91020242091460.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB100-56326-img0002.jpg "Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0006.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.](http://images.novusbio.com/fullsize/Tau-Antibody-2E9-Western-Blot-NBP2-25162-img0006.jpg "Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.](http://images.novusbio.com/fullsize/Tau-Antibody-2E9-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.](http://images.novusbio.com/fullsize/Tau-Antibody-2E9-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0004.jpg)
![Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NBP2-24390-img0002.jpg "Western Blot: SOX2 Antibody [NBP2-24390] - Analysis of SOX2 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-24390] - FFPE human skin stained with SOX2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunohistochemistry-Paraffin-NBP2-24390-img0003.jpg)