| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83952-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82601-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-542-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9355-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB1580-SP | |||
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human gastrointestinal, kidney, liver and testis using Anti-PCYT2 antibody NBP1-83952 (A) shows similar protein distribution across tissues to independent antibody NBP1-83951 (B).](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83952-img0014.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human gastrointestinal, kidney, liver and testis using Anti-PCYT2 antibody NBP1-83952 (A) shows similar protein distribution across tissues to independent antibody NBP1-83951 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: PCYT2 Antibody [NBP1-83952] - Analysis using Anti-PCYT2 antibody NBP1-83952 (A) shows similar pattern to independent antibody NBP1-83951 (B).](http://images.novusbio.com/fullsize/PCYT2-Antibody-Western-Blot-NBP1-83952-img0009.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83952-img0017.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83952-img0015.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83952-img0016.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83952-img0018.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Wild-type (WT) and HTT knockout (KO) 293T cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13106_rabbit-huntingtin-pab-161020241940576.jpg)
![Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT1553-RB-Western-Blot-NBP3-13658-img0001.jpg "Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![C9orf72 Antibody (1553-RB) [NBP3-13658] - DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [1553-RB] diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-immunocytochemistry-immunofluorescence-132202483135..jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-1610202419414565.jpg)
![Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-western-blot-2611202518243914.jpg)
![C9orf72 antibody [GT1553-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.C9orf72 stained by C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-immunohistochemistry-paraffin-2611202518321916.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13658_rabbit-c9orf72-mab-gt1553-rb-western-blot-2611202518381511.jpg)
![Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse nucleus ambiguus and lateral paragigantocellular nucleus shows positivity in neurons.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-NBP1-82601-img0009.jpg "Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse nucleus ambiguus and lateral paragigantocellular nucleus shows positivity in neurons.")
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of human testis shows strong cytoplasmic positivity in seminiferus ducts and leydig cells.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-82601-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of human lateral ventricle shows cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-82601-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining shows weak cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-82601-img0005.jpg)
![Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse caudate putamen shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-NBP1-82601-img0006.jpg)
![Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse midbrain shows neuronal positivity in the reticular nucleus.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-NBP1-82601-img0007.jpg)
![Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse motor cortex shows immunoreactivity in neuronal cell bodies and dendrites.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-NBP1-82601-img0008.jpg)
![Immunohistochemistry: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-82601] - Staining of mouse thalamus shows neuronal immunoreactivity in paraventricular thalamic nucleus.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-NBP1-82601-img0010.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-1A-Immunohistochemistry-Paraffin-NB300-542-img0002.jpg "Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.")
![Flow Cytometry: CACNA1S Antibody (1A) [NB300-542] - Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-1A-Flow-Cytometry-NB300-542-img0003.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Western blot analysis of extracts of various cell lines, using SR-B2/LIMPII/SR-B2 Rabbit mAb (NBP3-16779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-7D2P5-Western-Blot-NBP3-16779-img0004.jpg "Western Blot: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Western blot analysis of extracts of various cell lines, using SR-B2/LIMPII/SR-B2 Rabbit mAb (NBP3-16779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded human liver tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025208458.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded mouse kidney tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025209822.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded Human lung adenocarcinoma tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-8220252010447.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded human thyroid cancer tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-8220252010489.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded mouse spleen tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025209857.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (7D2P5) [NBP3-16779] - Immunohistochemistry analysis of LIMPII/SR-B2 in paraffin-embedded rat kidney tissue using LIMPII/SR-B2 Rabbit mAb at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16779_rabbit-limpii-sr-b2-mab-7d2p5-822025208418.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Western-Blot-NBP3-16524-img0003.jpg "Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded mouse liver using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded human liver cancer using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0001.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded mouse kidney tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512432081.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded rat brain tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435344.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-722025124532.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver cancer tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435325.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Immunohistochemistry-Paraffin-MAB1580-img0001.jpg "Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.")
![Simple Western: HSP27 Antibody (967050) [MAB1580] - MCF-7 cell lysate. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Simple-Western-MAB1580-img0002.jpg)