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![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0005.jpg "Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Immunocytochemistry-Immunofluorescence-NBP2-61841-img0003.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Flow-Cytometry-NBP2-61841-img0002.jpg)
![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against human *** (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0004.jpg)
![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0006.jpg)
![ELISA: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-ELISA-NBP2-61841-img0001.jpg)
![Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Western-Blot-NBP1-44270-img0009.jpg "Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.")
![Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-NBP1-44270-img0010.jpg)
![Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-29656-img0001.jpg "Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.")
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody (red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29656-img0004.jpg)
![Western Blot: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Western-Blot-NBP2-74117-img0001.jpg "Western Blot: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.")
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0004.jpg)
![Flow Cytometry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SERPINE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74117-img0002.jpg)
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0003.jpg)
![Immunohistochemistry: Serpin E2/PN1 Antibody (OTI2C9) - Azide and BSA Free [NBP2-74117] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINE2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Serpin-E2-PN1-Antibody-OTI2C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74117-img0005.jpg)
![Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Western-Blot-NBP2-70334-img0001.jpg "Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.")
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0004.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0002.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0003.jpg)
![Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Calpain-2-Antibody-OTI3G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70334-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer](http://images.novusbio.com/fullsize/RTN1-Antibody-MON162-Immunocytochemistry-Immunofluorescence-NBP1-97677-img0001.jpg "Immunocytochemistry/Immunofluorescence: RTN1 Antibody (MON162) [NBP1-97677] - Immunostaining of NCI-H82 variant small cell lung cancer")
![Immunohistochemistry-Frozen: RTN1 Antibody (MON162) [NBP1-97677] - Immunohistochemistry on frozen section of swine brain](http://images.novusbio.com/fullsize/RTN1-Antibody-MON162-Immunohistochemistry-Frozen-NBP1-97677-img0002.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0004.jpg "Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: KCNN2 Antibody [NBP2-84112] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN2. Gene Name: KCNN2. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-NBP2-84112-img0006.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - WB Suggested Anti-KCNN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0001.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0002.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0003.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target: KCNN2. Positive control (+): Human liver (LI). Negative control (-): HeLa (HL). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0005.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0002.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0001.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0002.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).")
![Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204] - Staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunohistochemistry-Frozen-NBP2-62204-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0001.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)
![Western Blot: PEN2 Antibody [NB300-227] - Analysis of PEN2 on human placenta.](http://images.novusbio.com/fullsize/PEN2-Antibody-Western-Blot-NB300-227-img0003.jpg "Western Blot: PEN2 Antibody [NB300-227] - Analysis of PEN2 on human placenta.")