| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74850 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92886-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB120-10505-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92684SS | |||
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518341523.jpg "Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518382820.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518435619.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-141120251841520.jpg)
![AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunocytochemistry-immunofluorescence-1411202518424111.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518312110.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518394118.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518334523.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202374820.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Analysis in human cerebral cortex and cervix, uterine tissues using NBP2-33774 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GFAP Antibody [NBP2-33774] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NBP2-33774] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33774-img0017.jpg)
![Western Blot: GFAP Antibody [NBP2-33774] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NBP2-33774-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human hippocampus shows positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0039.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-33774-img0040.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-33774] -Staining of human cervix, uterine shows no cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-33774_rabbit-polyclonal-gfap-antibody-immunohistochemistry-paraffin-166202375644..jpg)
![Western Blot: GFAP Antibody (OTI4D11) [NBP1-47782] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Western-Blot-NBP1-47782-img0001.jpg "Western Blot: GFAP Antibody (OTI4D11) [NBP1-47782] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.")
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded thyroid tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0016.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0002.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded bladder tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0004.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded Kidney tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0011.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded lung tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0012.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded lymph node tissue using anti-GFAP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0013.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (OTI4D11) [NBP1-47782] - Staining of paraffin-embedded prostate tissue using anti-GFAPmouse monoclonal antibody.](http://images.novusbio.com/fullsize/GFAP-Antibody-OTI4D11-Immunohistochemistry-Paraffin-NBP1-47782-img0015.jpg)
![Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - VMAT2 protein level by using anti-VMAT2 (9E11) antibody in the striatum of autopsied human brain.](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Western-Blot-NBP1-47977-img0008.jpg "Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - VMAT2 protein level by using anti-VMAT2 (9E11) antibody in the striatum of autopsied human brain.")
![Immunocytochemistry/Immunofluorescence: VMAT2 Antibody (OTI9E11) [NBP1-47977] - VMAT2 Antibody (9E11) [NBP1-47977] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VMAT2.](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Immunocytochemistry-Immunofluorescence-NBP1-47977-img0005.jpg)
![Immunohistochemistry-Paraffin: VMAT2 Antibody (9E11) [NBP1-47977] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Immunohistochemistry-Paraffin-NBP1-47977-img0003.jpg)
![Flow Cytometry: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VMAT2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Flow-Cytometry-NBP1-47977-img0001.jpg)
![Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Western-Blot-NBP1-47977-img0002.jpg)
![Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti SLC18A2 monoclonal antibody.(1:200).](http://images.novusbio.com/fullsize/VMAT2-Antibody-9E11-Western-Blot-NBP1-47977-img0006.jpg)
![Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Western-Blot-NBP2-02358-img0002.jpg "Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/APP-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0009.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded prostate tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0007.jpg)
![Flow Cytometry: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells transfected with either pCMV6-ENTRY Amyloid Precursor Protein.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Amyloid Precursor Protein mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Flow-Cytometry-NBP2-02358-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0003.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded colon tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0004.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded Kidney tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0005.jpg)
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0006.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Western Blot-NBP2-92886-img0003.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded mouse brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-42202514264496.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-4220251430473.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Western-Blot-NBP3-15642-img0005.jpg "Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-7B9C8-Immunohistochemistry-Paraffin-NBP3-15642-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517344224.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333895.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517304947.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517315522.jpg)
![Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517361214.jpg)
![Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-52202517333868.jpg)
![Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-15642_rabbit-huntingtin-mab-7b9c8-5220251734569.jpg)
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BDNF-Antibody-35928-11-Immunocytochemistry-Immunofluorescence-NB120-10505-img0001.jpg "Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.")
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0007.jpg "Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunohistochemistry-Paraffin-NBP2-67572-img0003.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0002.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Western-Blot-NBP1-92684-img0005.jpg "Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
