| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94464-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35164-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47607-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59330-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59378-25ug | |||
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0006.jpg "Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - analysis of CACNB2 in HEK293 and CHO whole cell lysates using anti-CACNB2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0004.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0005.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Primary antibody dilution-1:1000 in blocking buffer, 5% Milk in TBS-0.05% Tween for 2 hours at RT. Blocking Overnightat +4 degreeC 2 Hours at RT. Secondary antibody: 1:2000 in blocking buffer, 1 hourat RT. Image from verified customer review.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0007.jpg)
![ELISA: CACNB2 Antibody (6C4) [H00000783-M05] - Detection limit for recombinant GST tagged CACNB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-ELISA-H00000783-M05-img0003.jpg)
![Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518415223.jpg "<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518335915.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842516.jpg)
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251839419.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518392014.jpg)
![P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-immunocytochemistry-immunofluorescence-1411202518333113.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842412.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249393566.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024941475.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249415931.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024943286.jpg)
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody [NBP2-22458] - Immunolocalization of IP3 receptor type I in rat cerebellum.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22458-img0001.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody [NBP2-22458] - Immunolocalization of IP3 receptor type I in rat cerebellum.")
![Western Blot: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Analysis of extracts of various cell lines, using Syntenin 1 Rabbit mAb (NBP3-16603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Western-Blot-NBP3-16603-img0005.jpg "Western Blot: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Analysis of extracts of various cell lines, using Syntenin 1 Rabbit mAb (NBP3-16603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Mouse brain using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0003.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Rat brain using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0001.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Human colon using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0002.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (3J5O6) [Syntenin 1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Syntenin 1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16603_rabbit-syntenin-1-mab-3j5o6-52202517152745.jpg)
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0006.jpg "Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Rat kidney using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0004.jpg)
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of HepG2, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0005.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human breast cancer using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0002.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human liver using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0003.jpg)
![Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415143692.jpg "Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415133459.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using SERCA2 ATPase Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202414154330.jpg)
![Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody [NBP3-35164] - Immunofluorescence analysis of C6 cells using SERCA2 ATPase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415171187.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] - Immunohistochemistry analysis of paraffin-embedded Rat heart using SERCA2 ATPase Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-91220241421372.jpg)
![Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KIF3A-Antibody-Western-Blot-NBP3-05030-img0003.jpg "Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05030-img0001.jpg)
![Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunoprecipitation-NBP3-05030-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody [NBP3-05030] - Rat testis using KIF3A Rabbit pAb at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-05030_rabbit-polyclonal-kif3a-antibody-immunohistochemistry-paraffin-146202493146..jpg)
![Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Western-Blot-NBP1-47607-img0006.jpg "Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (NBP1-47607). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (NBP1-47607) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0008.jpg)
![Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunohistochemistry-NBP1-47607-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0003.jpg)
![Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Western-Blot-NBP2-59330-img0004.jpg "Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.")
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0006.jpg)
![Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Western-Blot-NBP2-59378-img0004.jpg "Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT/SLC18A3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0003.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)