| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47948-0.1mg | |||
| IHC-Prediluted | NBP2-48101 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21163-100ul | |||
| BSA Free | NBP3-21163-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03702-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92928-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59326-25ug | |||
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0005.jpg "Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (NB100-40828). Detection: DAB](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-NB100-40828-img0006.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Spectrin beta 2 Antibody [NB100-40828] - Detection of human Spectrin beta 2 (SPTBN1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Spectrin beta 2 antibody NB100-40828 used for IP at 3 ug per reaction. SPTBN1 was also immunoprecipitated by rabbit anti-Spectrin beta 2 antibody NB100-40829. For blotting immunoprecipitated SPTBN1, NB100-40828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunoprecipitation-NB100-40828-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 19 Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 19 Antibody (KRT19/799 + KRT19/800)")
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Bladder Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Ovarian Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0005.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Brain Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0007.jpg "Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Brain Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: GABA-A R rho 2 Antibody [NBP1-80061] - Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Membrane(tight junctions - intercalated disks) Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec Protocol located in Reviews and Data.](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Immunohistochemistry-NBP1-80061-img0004.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0002.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0005.jpg)
![Western Blot: GABA-A R rho 2 Antibody [NBP1-80061] - Sample Type: 293T Antibody Dilution: 1.0 ug/ml GABRR2 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Western-Blot-NBP1-80061-img0006.jpg)
![Immunohistochemistry: GABA-A R rho 2 Antibody [NBP1-80061] - Human Adult heart Observed Staining: Membrane (tight junctions - intercalated disks) Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 s](http://images.novusbio.com/fullsize/GABA-A-R-rho-2-Antibody-Immunohistochemistry-NBP1-80061-img0003.jpg)
![Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP3-03702-img0003.jpg "Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - WB image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using Glutathione Synthetase antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Knockout-Validated-NBP3-03702-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-52202519313410.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-52202519302977.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody - Azide and BSA Free [NBP3-03702] - Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase Rabbit pAb (A14535) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03702_rabbit-polyclonal-glutathione-synthetase-antibody-5220251931397.jpg)
![Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-simple-western-2042023113534.jpg "Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.")
![Western Blot: ATP5A Antibody [NBP2-92928] - Analysis of extracts of various cell lines, using ATP5A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-92928-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of Hela cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251430450.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of U-2OS cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251429052.jpg)
![Western Blot: Filamin A Antibody (3X8Y9) [NBP3-16266] - Western blot analysis of extracts of various cell lines, using Filamin A Rabbit mAb (NBP3-16266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-3X8Y9-Western-Blot-NBP3-16266-img0004.jpg "Western Blot: Filamin A Antibody (3X8Y9) [NBP3-16266] - Western blot analysis of extracts of various cell lines, using Filamin A Rabbit mAb (NBP3-16266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Filamin A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511392272.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Filamin A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511361268.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Filamin A Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511361276.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Filamin A Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-5220251242539.jpg)
![Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Western-Blot-NBP2-82069-img0001.jpg "Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-82069] - Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82069-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 ug/ml.Red: ABCA7 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120241958468.gif)
![Immunohistochemistry: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-2711202419592055.gif)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120242022969.jpg)
![Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Western-Blot-NBP2-59326-img0007.jpg "Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0004.jpg)
![Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-278202415242660.jpg "Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32247) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-298202413585528.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin 19 with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-29202410334065.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Flow cytometric analysis of MCF7 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32247, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-39202488462.jpg)
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47759-img0002.jpg "Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Human Stomach tissue stained with CCR5 Antibody (12D1).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47759-img0001.jpg)
![Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47759-img0003.jpg)