| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45345 | |||
| Azide and BSA Free | NBP2-34733-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78133SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57697-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82527-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41305-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70376 | |||
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0018.jpg "Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000")
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Mouse lung. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0014.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (30 ug of whole cell lysate) A:A43112% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0020.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGETMEM59 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0017.jpg)
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Human ovarian carcinoma. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0015.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM123-Immunohistochemistry-Paraffin-NBP2-32933-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)")
![Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-240-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-NB300-240-img0006.jpg)
![Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunoprecipitation-NB300-240-img0011.jpg)
![Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAML3-Antibody-Western-Blot-NB100-2129-img0005.jpg "Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2129). Detection: DAB](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-NB100-2129-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2129 (lot NB100-2129-2) used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2129-1) and rabbit anti-MAML3 antibody NB100-2128 For blotting immunoprecipitated MAML3, NB100-2129 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunoprecipitation-NB100-2129-img0007.jpg)
![Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Western-Blot-NBP2-78133-img0002.jpg "Western Blot: SCP3/SYCP3 Antibody [NBP2-78133] - Total protein from human and mouse testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SCP3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NBP2-78133] - IHC analysis of a FFPE tissue section of the mouse testis using 1:200 dilution of SCP3 antibody (NBP2-78133). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated strong staining associated with chromatin in meiotic cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NBP2-78133-img0001.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human cerebral cortex, hypothalamus, liver and testis using Anti-PMCH antibody NBP2-57697 (A) shows similar protein distribution across tissues to independent antibody NBP2-13780 (B).](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human cerebral cortex, hypothalamus, liver and testis using Anti-PMCH antibody NBP2-57697 (A) shows similar protein distribution across tissues to independent antibody NBP2-13780 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: PMCH Antibody [NBP2-57697] - Staining of mouse brain shows strong positivity in of neurons in the hypothalamus.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-NBP2-57697-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0002.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0003.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human hypothalamus.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0005.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of rat hypothalamus shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0006.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0008.jpg "Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry: NELL2 Antibody [NBP1-82527] - NELL2 expression in the 3% PFA perfused mouse brain Hippocampus and Cortex using primary antibody with 1:100 dilution. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-NBP1-82527-img0004.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human placenta shows very weak cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0005.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0007.jpg)
![Immunohistochemistry-Paraffin: NELL2 Antibody [NBP1-82527] - Staining of human Fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NELL2-Antibody-Immunohistochemistry-Paraffin-NBP1-82527-img0006.jpg)
![Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Western-Blot-NBP2-02779-img0006.jpg "Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (1A5) [NBP2-02779] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Immunocytochemistry-Immunofluorescence-NBP2-02779-img0005.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Coronin-1a antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0003.jpg)
![Western Blot: Coronin-1a Antibody (1A5) [NBP2-02779] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Western-Blot-NBP2-02779-img0004.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0001.jpg)
![Flow Cytometry: Coronin-1a Antibody (1A5) [NBP2-02779] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-1A5-Flow-Cytometry-NBP2-02779-img0002.jpg)
![Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/HES5-Antibody-Western-Blot-NBP2-41305-img0001.jpg "Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: HES5 Antibody - BSA Free [NBP2-41305] - Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-2711202420324837.gif)
![Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 ug/mL.Green: HES5 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-271120242011551.gif)
![Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-2711202420392744.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.")
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0007.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD38 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0002.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0004.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0005.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0006.jpg)
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0004.jpg "Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0002.jpg)
![Flow Cytometry: Coronin-1a Antibody [NB100-1298] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Flow-Cytometry-NB100-1298-img0005.jpg)
![Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB100-1298-img0003.jpg)
![Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunohistochemistry-Paraffin-NB100-1298-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)