| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68136SS | |||
| Azide and BSA Free | NBP2-80973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-542-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89639-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33541-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59617 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47920-0.1mg | |||
| IHC-Prediluted | NBP2-48457 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27774-50ul | |||
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Intracellular-NB110-68136-img0010.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![<b>Biological Strategies Validation. </b>Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-BSA-Free-Flow-Cytometry-NB110-68136-img0016.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0008.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0007.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0011.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0013.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0014.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-1A-Immunohistochemistry-Paraffin-NB300-542-img0002.jpg "Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.")
![Flow Cytometry: CACNA1S Antibody (1A) [NB300-542] - Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-1A-Flow-Cytometry-NB300-542-img0003.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-89639-img0002.jpg "Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.")
![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1012) - Azide and BSA Free [NBP2-47924] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942).](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1012-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47924-img0002.jpg "Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1012) - Azide and BSA Free [NBP2-47924] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942).")
![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1012) - Azide and BSA Free [NBP2-47924] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942).](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1012-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47924-img0001.jpg)
![Protein Array: S100A1 Antibody (S100A1/1012) - Azide and BSA Free [NBP2-47924] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A1 Mouse Monoclonal Antibody (S100A1/1942).](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1012-Azide-and-BSA-Free-Protein-Array-NBP2-47924-img0003.jpg)
![Western Blot: S100A1 Antibody (1D5) [H00006271-M01] - Analysis of S100A1 expression in transfected 293T cell line by S100A1 monoclonal antibody (M01), clone 1D5.Lane 1: S100A1 transfected lysate(10.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/S100A1-Antibody-1D5-Western-Blot-H00006271-M01-img0004.jpg "Western Blot: S100A1 Antibody (1D5) [H00006271-M01] - Analysis of S100A1 expression in transfected 293T cell line by S100A1 monoclonal antibody (M01), clone 1D5.Lane 1: S100A1 transfected lysate(10.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: S100A1 Antibody (1D5) [H00006271-M01] - Detection limit for recombinant GST tagged S100A1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/S100A1-Antibody-1D5-Sandwich-ELISA-H00006271-M01-img0003.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Analysis in human skeletal muscle and prostate tissues. Corresponding CACNA1S RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-Immunohistochemistry-Paraffin-NBP2-33541-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Analysis in human skeletal muscle and prostate tissues. Corresponding CACNA1S RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-Immunohistochemistry-Paraffin-NBP2-33541-img0008.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-Immunohistochemistry-Paraffin-NBP2-33541-img0005.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Staining of human prostate shows no positivity in smooth muscle cells as expected.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-Immunohistochemistry-Paraffin-NBP2-33541-img0006.jpg)
![Immunohistochemistry-Paraffin: CACNA1S Antibody [NBP2-33541] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CACNA1S-Antibody-Immunohistochemistry-Paraffin-NBP2-33541-img0007.jpg)
![Protein Array: S100A1 Antibody (S100A1/1942) [NBP2-59615] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1942-Protein-Array-NBP2-59615-img0003.jpg "Protein Array: S100A1 Antibody (S100A1/1942) [NBP2-59615] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) [NBP2-59615] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Antibody (S100A1/1942).](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1942-Immunohistochemistry-Paraffin-NBP2-59615-img0004.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) [NBP2-59615] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942)](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1942-Immunohistochemistry-Paraffin-NBP2-59615-img0001.jpg)
![Western Blot: S100A/B Antibody (C8) [NBP3-41140] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate](http://images.novusbio.com/fullsize/nbp3-41140_mouse-s100a-b-mab-c8-6122024212749.jpg "Western Blot: S100A/B Antibody (C8) [NBP3-41140] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate")
![Immunohistochemistry-Paraffin: S100A/B Antibody (C8) [NBP3-41140] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30ug/ml NBP3-41140; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41140_mouse-s100a-b-mab-c8-612202421283820.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (SPM319) [NBP2-44583] - Human lung carcinoma stained with Tenascin C monoclonal antibody (SPM319) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-SPM319-Immunohistochemistry-Paraffin-NBP2-44583-img0001.jpg "Immunohistochemistry-Paraffin: Tenascin C Antibody (SPM319) [NBP2-44583] - Human lung carcinoma stained with Tenascin C monoclonal antibody (SPM319) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.")
![Western Blot: S100A/B Antibody (SR3487) [NBP3-27774] - Western blot analysis of (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27774_rabbit-s100a-b-mab-sr3487-75202410133418.jpg "Western Blot: S100A/B Antibody (SR3487) [NBP3-27774] - Western blot analysis of (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.")
![Immunohistochemistry-Paraffin: S100A/B Antibody (SR3487) [NBP3-27774] - Immunohistochemical analysis of paraffin-embedded human kidney](http://images.novusbio.com/fullsize/nbp3-27774_rabbit-s100a-b-mab-sr3487-75202410133478.jpg)