| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-402SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36573SS | |||
| Azide and BSA Free | NBP2-80951 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11863SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44128 | |||
| BSA Free | NBP2-59061-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15874-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37528-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-402-img0004.jpg "Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [NB100-402] - CTR1 antibody was tested in NIH-3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen competition in breast cancer tissue.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-NB100-402-img0002.jpg)
![Immunohistochemistry: SLC31A1/CTR1 Antibody [NB100-402] - Left panel: Antibody staining of hCTR1 overexpressing cell line. Right panel: Antibody plus peptide competition staining.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-NB100-402-img0003.jpg)
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)
![Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-36573-img0009.jpg)
![Immunohistochemistry: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - IL-17 induces cellular copper uptake through induction of STEAP4. Western blots analysis for copper trafficking-associated proteins from lysates of mouse colon epithelial organoids treated with different inflammatory cytokines for 8h. Three independent experiments were done and representative blots were shown. Untr untreated. IL-17 primed or unprimed mouse colon organoids were stained for STEAP4 (St4), E-cadherin (E-cad) and SLC31A1/CTR1 and integrin alpha. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14698-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-NBP2-36573-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - STEAP4 promotes colitis-associated colon tumorigenesis. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32060280/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0011.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Aanalysis of a FFPE tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0007.jpg)
![Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Western-Blot-NB300-132-img0003.jpg "Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Immunocytochemistry-Immunofluorescence-NB300-132-img0004.jpg)
![Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Simple-Western-NB300-132-img0005.jpg)
![Western Blot: TET1 Antibody [NBP3-11863] - Total protein from human K562 Nuclear and mouse L929 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TET1 (NBP3-11863) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/TET1-Antibody-Western-Blot-NBP3-11863-img0001.jpg "Western Blot: TET1 Antibody [NBP3-11863] - Total protein from human K562 Nuclear and mouse L929 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TET1 (NBP3-11863) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - PC12 cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0007.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP3-11863] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TET1 antibody (NBP3-11863). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP3-11863-img0002.jpg)
![Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on NIH3T3 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/TET1-Antibody-Flow-Intracellular-NBP3-11863-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - HeLa cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP3-11863] - NIH3T3 cells were fixed for 10 minutes using 4% paraformaldehyde and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TET1 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11863-img0006.jpg)
![Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on A431 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/TET1-Antibody-Flow-Intracellular-NBP3-11863-img0008.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0023.jpg "Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.")
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - Representative western blots and densitometry analysis of TET1 levels in untreated or DAC-treated (a) HCT116 monocultures and cocultures with normal BJ or sBJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody (GT1462) [NBP2-15135] - detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TET1-Antibody-GT1462-Immunocytochemistry-Immunofluorescence-NBP2-15135-img0011.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - Mouse testis. TET1 stained by TET1 antibody [1462] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Immunohistochemistry-Paraffin-NBP2-15135-img0027.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody (GT1462) [NBP2-15135] - A. 50 ug whole cell lysate/extract from 293T cells transfected with scramble siRNA . B. 50 ug whole cell lysate/extract from TET1-knockdowned 293T cells.](http://images.novusbio.com/fullsize/TET1-Antibody-GT1462-Western-Blot-NBP2-15135-img0013.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody dilution: 1:500](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Western-Blot-NBP2-15135-img0025.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - HeLa xenograft. TET1 antibody [1462] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TET1-Antibody-1462-Immunohistochemistry-Paraffin-NBP2-15135-img0022.jpg)
![Western Blot: TET1 Antibody (1462) [NBP2-15135] - Changes in the expression of DNMT1 & TET1. Representative western blots & densitometry analysis of DNMT1 & TET1 levels in untreated or DAC-treated (a) HCT116 monocultures & cocultures with normal BJ or sBJ & (b) HCT116 monocultures grown in conditioned medium from normal BJ or sBJ cells. DNMT1 & TET1 blots in (a) & (b) are taken from different gels. Data are mean ± SEM, n = 3, ∗∗∗p < 0.001, ∗p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15135_mouse-monoclonal-tet1-antibody-1462-31020241538416.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-910202420122065.jpg "Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (NBP2-76345) dilution: 1:1000.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242063370.jpg)
![Western Blot: TET1 Antibody [NBP2-76345] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242011503.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020241957212.jpg)
![Immunohistochemistry: TET1 Antibody [NBP2-76345] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (NBP2-76345) antibody at 1:1000 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242014666.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242021956.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (NBP2-76345) diluted at 1:250.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242091468.jpg)
![Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP2-76345] - TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 stained by TET1 antibody [N3C1] (NBP2-76345) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-91020242041232.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET1 Antibody [NBP2-76345] - Non-transfected (–) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-1610202419414597.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-western-blot-2611202518384013.jpg)
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (NBP2-76345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-76345_rabbit-polyclonal-tet1-antibody-western-blot-261120251849398.jpg)
![Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Western-Blot-NBP2-59061-img0008.jpg "Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061] - Staining of PC-3 cells using the Anti-NEFL monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunocytochemistry-Immunofluorescence-NBP2-59061-img0010.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0009.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0001.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0003.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0004.jpg)
![Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-NBP2-59061-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human liver shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/NF-L-Antibody-CL4729-Immunohistochemistry-Paraffin-NBP2-59061-img0007.jpg)
![Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-271120242022977.jpg "Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-271120242004193.jpg)
![Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202419592040.jpg)
![Western Blot: TET1 Antibody [NBP3-27892] - Figure 3 WB Validation of TET1 in Rat Tissues Loading: 15 u of lysate Antibodies: TET1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202420333867.gif)
![Western Blot: TET1 Antibody [NBP3-27892] - Figure 2 WB Validation of TET1 in Mouse Tissues Loading: 15 u of lysate Antibodies: TET1 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202420392785.gif)
![Western Blot: TET1 Antibody [NBP3-27892] - Figure 1 WB Validation of TET1 in Human Cell Lines Loading: 15 u of lysate Antibodies: TET1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202419172212.gif)
![Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] - Figure 5Immunofluorescence Validation of TET1 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202419293896.gif)
![Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] - Figure 4 Immunofluorescence Validation of TET1 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp3-27892_rabbit-tet1-pab-2711202419333458.gif)
![Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235717.jpg "Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235788.jpg)
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517152736.jpg)
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-5220251721355.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517172848.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of Human brain tissue using NF-L Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517162336.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Mouse brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517184520.jpg)
![Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Western-Blot-NBP2-37528-img0003.jpg "Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (1H3) [NBP2-37528] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunocytochemistry-Immunofluorescence-NBP2-37528-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (1H3) [NBP2-37528] - Analysis of bladder cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunohistochemistry-NBP2-37528-img0007.jpg)
![Flow Cytometry: NF-L Antibody (1H3) [NBP2-37528] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Flow-Cytometry-NBP2-37528-img0005.jpg)
![Western Blot: NF-L Antibody (1H3) [NBP2-37528] - Analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Western-Blot-NBP2-37528-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (1H3) [NBP2-37528] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-Immunohistochemistry-NBP2-37528-img0006.jpg)
![ELISA: NF-L Antibody (1H3) [NBP2-37528] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-1H3-ELISA-NBP2-37528-img0001.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)