| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92713-50uL | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22190-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26573-50ul | |||
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human liver and pancreas tissues. Corresponding APOE RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human liver and pancreas tissues. Corresponding APOE RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cerebral cortex, liver, pancreas and testis using Anti-APOE antibody NBP2-49450 (A) shows similar protein distribution across tissues to independent antibody NBP2-49565 (B).](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0009.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Analysis in human plasma.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP2-49450-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49450-img0008.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0015.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0012.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0013.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP2-49450] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP2-49450-img0014.jpg)
![Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Western-Blot-NBP1-92713-img0002.jpg "Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713] - Mixed neuronal cultures stained with GAP-43 antibody (1E3) (green), a rabbit antibody to microtubule associated protein 2 (red) and DNA (blue). The GAP-43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP1-92713-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Complement C3d Antibody (C3D/2891) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Immunohistochemistry-Paraffin-NBP3-07741-img0003.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Complement C3d Antibody (C3D/2891) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Formalin-fixed, paraffin-embedded human rejected kidney transplant stained with Complement C3d Antibody (C3D/2891) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Immunohistochemistry-Paraffin-NBP3-07741-img0002.jpg)
![Protein Array: Complement C3d Antibody (C3D/2891) [NBP3-07741] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement C3d Mouse Monoclonal Antibody (C3D/2891).](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-C3D-2891-Protein-Array-NBP3-07741-img0001.jpg)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171] - Staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glycine-Receptor-Alpha-1-Antibody-Western-Blot-NBP2-26171-img0001.jpg "Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171] - Staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.](http://images.novusbio.com/fullsize/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.")
![<b>Biological Strategies Validation. </b>Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of Rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.](http://images.novusbio.com/fullsize/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0002.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0006.jpg "Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP2-22190-img0001.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22190-img0002.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0004.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0005.jpg)
![ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-ELISA-NBP2-22190-img0003.jpg)
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Western-Blot-NBP2-67463-img0008.jpg "Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0002.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0006.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution.<br/><br/> Lane 1: HT-29 cell lysate<br/> Lane 2: A549 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution.<br/><br/> Lane 1: HT-29 cell lysate<br/> Lane 2: A549 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58874-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of SH-SY5Y cells stained with rabbit pAb to UCHL1, NB110-58874, dilution 1:1,000 in green, and costained with mouse mAb to fibrillarin, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrilarin antibody specifically labels nucleoli.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0005.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB110-58874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0003.jpg)
![Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB110-58874-img0004.jpg)
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845324.jpg "Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845328.jpg)
![Western Blot: c-Fos Antibody (14C10) [NBP3-26573] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: c-Fos Antibody at 0.81ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 29, 37 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202411351611.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (14C10) [NBP3-26573] - Staining of HepG2 cells with c-Fos Antibody (14C10) at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-28220241249368.jpg)
![Flow Cytometry: c-Fos Antibody (14C10) [NBP3-26573] - Overlay histogram showing Hela cells stained with c-Fos Antibody (14C10) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202414183668.jpg)