| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94464-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95200-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35164-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61659-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05182-0.025mg | |||
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0006.jpg "Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Rat kidney using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0004.jpg)
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of HepG2, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0005.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human breast cancer using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0002.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human liver using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0003.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of extracts of various cell lines, using NFkB p100/p52 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-95200-img0005.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of extracts of various cell lines, using NFkB p100/p52 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NBP2-95200] - Analysis of U2OS cells using NFkB p100/p52 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95200-img0001.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Rat brain using NF-kB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Human breast using NF-KB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP2-95200] - Mouse testis using NF-kB2 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP2-95200-img0003.jpg)
![Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415143692.jpg "Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: SERCA2 ATPase Antibody [NBP3-35164] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit pAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415133459.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using SERCA2 ATPase Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202414154330.jpg)
![Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody [NBP3-35164] - Immunofluorescence analysis of C6 cells using SERCA2 ATPase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-912202415171187.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody [NBP3-35164] - Immunohistochemistry analysis of paraffin-embedded Rat heart using SERCA2 ATPase Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35164_rabbit-serca2-atpase-pab-91220241421372.jpg)
![Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-912202414154378.jpg "Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251744416.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251704787.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-3112025173144.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-immunohistochemistry-paraffin-1352025122932..png)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-181202616152120.jpg "Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]")
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261619287.jpg)
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261615213.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-18120261618187.jpg)
![Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunofluorescence-181202616154110.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0004.jpg "Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of Hela cells using DNM1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Immunocytochemistry-Immunofluorescence-NBP2-37309-img0005.jpg)
![Flow Cytometry: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of HEK293 cells using DNM1L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Flow-Cytometry-NBP2-37309-img0006.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against human DNM1L recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0002.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against HEK293 (1) and DNM1L (AA: 69-213)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0003.jpg)
![ELISA: DRP1 Antibody (4A4C7) [NBP2-37309] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-ELISA-NBP2-37309-img0001.jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0004.jpg "Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Immunocytochemistry-Immunofluorescence-NBP2-61659-img0003.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0005.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0002.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0001.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0006.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-32-BSA-Free-ELISA-NBP1-05182-img0001.jpg "ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.")
![Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-288202412461645.jpg "Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32933) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29820241420927.jpg)
![Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunocytochemistry analysis of TT cells labeling Secretagogin with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29202410334089.jpg)
![Flow Cytometry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Flow cytometric analysis of TT cells labeling Secretagogin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32933, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-392024884687.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0004.jpg "Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.")
![Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Immunohistochemistry-Paraffin-NBP2-67555-img0005.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0002.jpg)
![Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on A431 lysates using anti-Erk1(pT202/pY204)+Erk2(pT185/pY187) antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-Western-Blot-NBP2-67555-img0001.jpg)
![ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of Erk1 (pT202/pY204) + Erk2 (pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with lambda Protein Phosphatase whole cell lysates](http://images.novusbio.com/fullsize/ERK1-2-p-Thr202-p-Tyr204-p-Thr185-p-Tyr187-Antibody-SC58-01-NBP2-67555-img0003.jpg)