| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54399-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59779 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05555-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
![Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Western-Blot-NBP2-53271-img0002.jpg "Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) [NBP2-53271] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Immunohistochemistry-Paraffin-NBP2-53271-img0001.jpg)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NBP3-05555-img0002.jpg "Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.")
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555] - Immunofluorescent analysis of rat brain section stained with Tyrosine Hydroxylase Antibody at a dilution of 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Tyrosine Hydroxylase Antibody stains the dopaminergic TH-positive neurons and their processes of the substantia nigra.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05555-img0001.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0018.jpg "Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000")
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Mouse lung. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0014.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (30 ug of whole cell lysate) A:A43112% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0020.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGETMEM59 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0017.jpg)
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Human ovarian carcinoma. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0015.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0011.jpg "Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0008.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0006.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGE Ataxin 3 antibody dilution: 1:20000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0007.jpg)
![Immunohistochemistry-Paraffin: NDUFA12 Antibody [NBP1-32745] - NDUFA12 Antibody detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 Antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NDUFA12-Antibody-Immunohistochemistry-Paraffin-NBP1-32745-img0009.jpg "Immunohistochemistry-Paraffin: NDUFA12 Antibody [NBP1-32745] - NDUFA12 Antibody detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 Antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: NDUFA12 Antibody [NBP1-32745] - Cal27 xenograft, using NDUFA12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NDUFA12-Antibody-Immunohistochemistry-Paraffin-NBP1-32745-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFA12 Antibody [NBP1-32745] - Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NDUFA12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32745-img0008.jpg)
![Western Blot: NDUFA12 Antibody [NBP1-32745] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (NBP1-32745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32745_rabbit-polyclonal-ndufa12-antibody-91020242021987.jpg)
![Western Blot: NDUFA12 Antibody [NBP1-32745] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (NBP1-32745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32745_rabbit-polyclonal-ndufa12-antibody-9102024205640.jpg)
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RPB2-Antibody-HL1043-Western-Blot-NBP3-13698-img0001.jpg "Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-91020242021981.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-91020242024614.jpg)
![Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-91020242091415.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-910202419593523.jpg)
![Immunohistochemistry: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-9102024201146.jpg)
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-91020242075990.jpg)
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-910202420104439.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1043) [NBP3-13698] - RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.RPB2 stained by RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-9102024205617.jpg)
![Western Blot: RPB2 Antibody (HL1043) [NBP3-13698] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (NBP3-13698) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13698_rabbit-rpb2-mab-hl1043-910202420122047.jpg)
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RPB2-Antibody-HL1042-Western-Blot-NBP3-13697-img0001.jpg "Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - 293T whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-9102024201468.jpg)
![Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-9102024209143.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-91020242021996.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-910202420104462.jpg)
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-9102024204129.jpg)
![Immunohistochemistry-Paraffin: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-9102024201464.jpg)
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-91020242001054.jpg)
![Western Blot: RPB2 Antibody (HL1042) [NBP3-13697] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-91020242063373.jpg)
![Immunohistochemistry: RPB2 Antibody (HL1042) [NBP3-13697] - RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1042] (NBP3-13697) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC](http://images.novusbio.com/fullsize/nbp3-13697_rabbit-rpb2-mab-hl1042-910202420122070.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249415947.jpg "Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941595.jpg)
![Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941493.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249422671.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024943554.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)