| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-136-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33062 | |||
| IHC-Prediluted | NBP2-48235 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50612-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82249-0.025ml | |||
![Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420243341.jpg "Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![Western Blot: CRBN Antibody [NBP2-82079] - Analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP2-82079-img0001.jpg)
![Immunohistochemistry: CRBN Antibody [NBP2-82079] - Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunohistochemistry-NBP2-82079-img0002.jpg)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Human Cells. Loading: 15 ug of cell lysate Antibodies: CRBN, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-271120242013238.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Mouse Tissues.Loading: 15 ug of mouse tissue lysate Antibodies: CRBN, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420141976.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Rat Tissues. Loading: 15 ug of rat tissue lysate Antibodies: CRBN, 0.5 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202419592026.gif)
![Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP2-82079] - Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82079-img0003.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87759 (A) shows similar protein distribution across tissues to independent antibody NBP1-87760 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NFkB p100/p52 Antibody [NBP1-87759] - Western blot analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87759-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0012.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex using Anti-NFKB2 antibody.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0015.jpg)
![Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP2-25163-img0005.jpg "Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-tau, NBP2-25163, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The NBP2-25163 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25163-img0004.jpg)
![Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Western-Blot-NB300-136-img0002.jpg "Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.")
![Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Immunohistochemistry-NB300-136-img0001.jpg)
![Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Western-Blot-NBP2-29435-img0008.jpg "Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).")
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0007.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0006.jpg)
![Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NBP2-50612-img0002.jpg "Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50612-img0001.jpg)
![Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0003.jpg "Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of adult rat frontal cortex section stained with mouse neurofilament NF-M mAb, dilution 1:5,000 (Green), and costained with chicken neurofilament NF-H pAb, dilution 1:5,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The mouse NF-M mAb labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-M pAb stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Immunohistochemistry-Free-Floating-NBP2-31206-img0004.jpg)
![Western Blot: Neurofilament NF-M Antibody (MCA-3H11) [NBP2-31206] - Cytoskeletal homogenate of rat spinal cord was separated by 7.5% SDS-PAGE. Lane A: Western blot analysis of Neurofilament NF-M with NBP2-31206. Lane B: SDS-PAGE gel stained with Commassie Brilliant Blue. The bands corresponding to Neurofilament subunits NF-H, NF-M, NF-L, a-internexin and GFAP are indicated.](http://images.novusbio.com/fullsize/Neurofilament-NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0002.jpg)
![Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Western-Blot-NB100-82249-img0002.jpg "Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.")
![Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr231) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Immunohistochemistry-NB100-82249-img0001.jpg)
![Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB100-41097-img0003.jpg "Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody [NB100-41097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41097-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0005.jpg "Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-1820247526..jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] -Analysis of Neuro-2a cells labeling Phospho-alpha Synuclein with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-immunocytochemistry-immunofluorescence-18202475444..jpg)
![Flow Cytometry:alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of HeLa cells labeling Phospho-alpha Synuclein (S129).Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-75409_rabbit-monoclonal-alpha-synuclein-antibody-jb22-44-flow-cytometry-18202475655..jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. Beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut3-Antibody-JA50-31-Western-Blot-NBP2-66872-img0007.jpg "Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. Beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.")
![Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Glut3-Antibody-JA50-31-Flow-Cytometry-NBP2-66872-img0005.jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)Lane 2: RAW264.7 cell lysate (20 ug/Lane)Lane 3: C6 cell lysate (20 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66872_rabbit-monoclonal-glut3-antibody-ja50-31-western-blot-6620249415..jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution.Lane 1: HepG2-si NT cell lysate (10 ug/Lane)Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute; ECL: merk4-20% SDS-PAGE gel.NBP2-66872 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66872_rabbit-monoclonal-glut3-antibody-ja50-31-western-blot-6620249631..jpg)
![Western Blot: MT-CO2 Antibody [NBP2-37945] - Analysis using Anti-MT-CO2 antibody NBP2-37945 (A) shows similar pattern to independent antibody NBP2-37930 (B).](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Western-Blot-NBP2-37945-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MT-CO2 Antibody [NBP2-37945] - Analysis using Anti-MT-CO2 antibody NBP2-37945 (A) shows similar pattern to independent antibody NBP2-37930 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human stomach shows strong granular cytoplasmic positivity in parietal cells.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0015.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining in human heart muscle and lymph node tissues using anti-MT-CO2 antibody. Corresponding MT-CO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0006.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human kidney.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0009.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human liver.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human cerebellum, kidney, liver and stomach using Anti-MT-CO2 antibody NBP2-37945 (A) shows similar protein distribution across tissues to independent antibody NBP2-37930 (B).](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0012.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0013.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0014.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody [NBP2-37945] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-Immunohistochemistry-Paraffin-NBP2-37945-img0016.jpg)