| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92187-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43791 | |||
| BSA Free | NBP2-46621-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43801 | |||
| BSA Free | NBP2-46626-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38300-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96141SS | |||
| Azide and BSA Free | NBP2-80665 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05209-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Analysis in human small intestine and lymph node tissues using HPA059299 antibody. Corresponding MAOA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Analysis in human small intestine and lymph node tissues using HPA059299 antibody. Corresponding MAOA RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MAO-A Antibody [NBP2-38868] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Western-Blot-NBP2-38868-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MAO-A Antibody [NBP2-38868] - Staining of human cell line RT4 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38868-img0005.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0006.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0007.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0008.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0009.jpg)
![Western Blot: NMDARA1 Antibody [NBP1-92187] - Analysis in control (vector only transfected HEK293T lysate) and GRINA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NMDARA1-Antibody-Western-Blot-NBP1-92187-img0007.jpg "Western Blot: NMDARA1 Antibody [NBP1-92187] - Analysis in control (vector only transfected HEK293T lysate) and GRINA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: NMDARA1 Antibody [NBP1-92187] - Staining of human cell line MCF7 shows localization to nucleoli fibrillar center.](http://images.novusbio.com/fullsize/NMDARA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92187-img0005.jpg)
![Immunohistochemistry-Paraffin: NMDARA1 Antibody [NBP1-92187] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NMDARA1-Antibody-Immunohistochemistry-Paraffin-NBP1-92187-img0006.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0008.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue")
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251 AAb](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0007.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining in human cerebral cortex and kidney tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining in human cerebral cortex and kidney tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody (CL2720) [NBP2-46626] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Western-Blot-NBP2-46626-img0007.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of rat brain shows moderate positivity in astrocytes in the hippocampus.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0016.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of mouse brain shows moderate positivity in the cerebellum.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human cerebral cortex shows moderate nuclear positivity in astrocytes.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human cerebellum shows strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0010.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (CL2720) [NBP2-46626] - Staining of human kidney shows no nuclear positivity as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0011.jpg)
![Immunohistochemistry: S100B Antibody (CL2720) [NBP2-46626] - Staining of rat brain shows strong positivity in glial cells in the cerebellum.](http://images.novusbio.com/fullsize/S100B-Antibody-CL2720-Immunohistochemistry-Paraffin-NBP2-46626-img0015.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum, colon, kidney and liver using Anti-LRPPRC antibody NBP2-38300 (A) shows similar protein distribution across tissues to independent antibody NBP1-83349 (B).](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum, colon, kidney and liver using Anti-LRPPRC antibody NBP2-38300 (A) shows similar protein distribution across tissues to independent antibody NBP1-83349 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: LRPPRC Antibody [NBP2-38300] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP2-38300-img0007.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human liver.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0011.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human colon.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0009.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0010.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human kidney.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0012.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg "Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-32744-img0011.jpg "Western Blot: LRPPRC Antibody [NBP1-32744] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-32744] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32744-img0010.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP1-32744] - Paraffin-embedded human colon cancer. LRP130 antibody [N3C2], Internal diluted at 1:100](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP1-32744-img0009.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] - Sample (30 ug of whole cell lysate) A: H1299 B: HeLa 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-32744-img0007.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP1-32744] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP1-32744-img0005.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] -Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32744_rabbit-polyclonal-lrpprc-antibody-western-blot-16202311429..jpg)
![Western Blot: ChABC Antibody (1E10) [NBP1-96141] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.](http://images.novusbio.com/fullsize/ChABC-Antibody-1E10-Western-Blot-NBP1-96141-img0001.jpg "Western Blot: ChABC Antibody (1E10) [NBP1-96141] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.")
![Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0005.jpg "Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-31684] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31684-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0007.jpg)
![Western Blot: NDUFB5 Antibody [NBP1-31684] - 50 ug mouse brain lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0004.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0003.jpg)
![Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684] - NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NDUFB5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Frozen-Negative-NBP1-31684-img0006.jpg)
![Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Western-Blot-NBP1-05209-img0005.jpg "Western Blot: NF-H Antibody (AH1) [NBP1-05209] - Analysis of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody (NBP1-05209) at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord. Rodent pNF-H protein appears as a single band of about 200kDa in adult rat and mouse lysates, but is not present in early development (Lane 3). Additional bands appearing on the blot (Lane 4) are most likely partially degraded products of pNF-H protein.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (AH1) [NBP1-05209] - Mixed neuron/glial cultures stained with NBP1-05209 (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L NB300-131 (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NBP1-05209 binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (AH1) [NBP1-05209] - Analysis of rat cerebellum section stained with mouse mAb to pNF-H, NBP1-05209, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05209 antibody stains axons in the granular layer and white matter and prominent basket cell axons surrounding the large Purkinje neurons. The FOX3/NeuN antibody specifically labels nuclei of granular and other neurons, but does not stain Purkinje cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-AH1-Immunocytochemistry-Immunofluorescence-NBP1-05209-img0004.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![<b>Biological Strategies Validation. </b>Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)