| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96141SS | |||
| Azide and BSA Free | NBP2-80665 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34808-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34206-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78784SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
![Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32426-img0011.jpg "Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0010.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0007.jpg)
![ELISA: Monoamine Oxidase B Antibody [NBP1-32426] - ELISA detection of MAOB using Monoamine Oxidase B antibody [N2C3] for capture at a concentration of 2.5 ug/mL and monoamine oxidase B for detection at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-ELISA-NBP1-32426-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-910202420134011.jpg)
![<b>Orthogonal Strategies Validation. </b>HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-141120251843448.jpg)
![HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-261120251854115.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: ChABC Antibody (1E10) [NBP1-96141] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.](http://images.novusbio.com/fullsize/ChABC-Antibody-1E10-Western-Blot-NBP1-96141-img0001.jpg "Western Blot: ChABC Antibody (1E10) [NBP1-96141] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.")
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Western-Blot-NBP2-32896-img0003.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Immunofluorescence of skin innervation using anti-PGP9.5. Antibody at dilution 1:500. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Immunocytochemistry-Immunofluorescence-NBP2-32896-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0012.jpg "Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Immunohistochemistry-Paraffin-H00004899-M01-img0007.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0008.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in HeLa.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0010.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in human Skeletal muscle.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0011.jpg)
![ELISA: Nrf1 Antibody (2F9) [H00004899-M01] - Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-ELISA-H00004899-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: OPA1 Antibody [NBP2-34206] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34206-img0006.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0007.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0009.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0010.jpg)
![Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-78784-img0003.jpg "Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TMEM49 Antibody [NBP2-78784] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TMEM49 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78784-img0001.jpg)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP2-78784] - Analysis of a FFPE tissue section of the human testis using 1:200 dilution of TMEM49 antibody (NBP2-78784). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in cytoplasm and membrane in seminiferous tubules.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP2-78784-img0002.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-32744-img0011.jpg "Western Blot: LRPPRC Antibody [NBP1-32744] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-32744] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32744-img0010.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP1-32744] - Paraffin-embedded human colon cancer. LRP130 antibody [N3C2], Internal diluted at 1:100](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP1-32744-img0009.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] - Sample (30 ug of whole cell lysate) A: H1299 B: HeLa 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-32744-img0007.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP1-32744] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP1-32744-img0005.jpg)
![Western Blot: LRPPRC Antibody [NBP1-32744] -Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32744_rabbit-polyclonal-lrpprc-antibody-western-blot-16202311429..jpg)
![Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0005.jpg "Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-31684] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31684-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0007.jpg)
![Western Blot: NDUFB5 Antibody [NBP1-31684] - 50 ug mouse brain lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Western-Blot-NBP1-31684-img0004.jpg)
![Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Paraffin-NBP1-31684-img0003.jpg)
![Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684] - NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NDUFB5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NDUFB5-Antibody-Immunohistochemistry-Frozen-Negative-NBP1-31684-img0006.jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025124758..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunohistochemistry-3042025123354..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunocytochemistry-immunofluorescence-3042025125627..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025125836..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513045..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513345..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513540..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025132854..jpg)
![<b>Orthogonal Strategies Validation. </b>Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] (NBP3-45160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-141120251831211.jpg)
![Tau antibody [HL2736] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2736] (NBP3-45160) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-frozen-14112025185543.jpg)