| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73786 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59503SS | |||
| Azide and BSA Free | NBP2-80971 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47893-0.1mg | |||
| IHC-Prediluted | NBP2-48117 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74436 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71988 | |||
![Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Western-Blot-NBP2-37626-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunocytochemistry-Immunofluorescence-NBP2-37626-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-Paraffin-NBP2-37626-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-NBP2-37626-img0004.jpg)
![ELISA: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-ELISA-NBP2-37626-img0001.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.](http://images.novusbio.com/fullsize/FoxM1-Antibody-Western-Blot-NBP1-30961-img0013.jpg "Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.")
![Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Representative images of immunoreactivity of nuclear FOXM1 in (I) benign cystadenoma, (II) borderline tumour, (III) stage I invasive cancer, (IV) stage II invasive cancer, (V) stage III invasive cancer and (VI) stage IV invasive cancer. Magnifications X400. Insets: Regions with higher magnifications of nuclear FOXM1 staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0113478), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FoxM1-Antibody-Immunohistochemistry-Paraffin-NBP1-30961-img0014.jpg)
![Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/H2AX-Antibody-Western-Blot-NBP2-27428-img0004.jpg "Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0007.jpg)
![Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27428] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunohistochemistry-Paraffin-NBP2-27428-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0006.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0023.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCM1 Antibody [NBP1-87196] - Staining of human cell line U-251 MG shows localization to nuclear membrane & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87196-img0018.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0015.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human ovary.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0021.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0022.jpg)
![Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Western-Blot-NBP2-03644-img0002.jpg "Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NBP2-03644-img0014.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0013.jpg)
![Flow Cytometry: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Flow-Cytometry-NBP2-03644-img0001.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0006.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0007.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0009.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0011.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0005.jpg "Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TPX2 Antibody [NB500-184] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-184. TPX2 was also immunoprecipitated using NB500-183 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels. TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels Depletion of TPX2 by siRNA causes a decrease in H4K16ac levels whereas the levels of H3K9ac and H3K56ac remain unchanged. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Knockdown-Validated-NB500-184-img0004.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416182636.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416161844.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416171433.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416173534.jpg)
![Simple Western: Survivin Antibody (1277A) [NBP2-59503] - Lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 10 ug/mL of Rabbit Anti-Human Monoclonal Survivin Antibody [NBP2-59503]. This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Simple-Western-NBP2-59503-img0002.jpg "Simple Western: Survivin Antibody (1277A) [NBP2-59503] - Lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 10 ug/mL of Rabbit Anti-Human Monoclonal Survivin Antibody [NBP2-59503]. This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.")
![Immunohistochemistry-Paraffin: Survivin Antibody (1277A) [NBP2-59503] - Analysis of a formalin-fixed paraffin-embedded (FFPE) human prostate cancer tissue with rabbit monoclonal Survivin Antibody [NBP2-59503] at 2 ug/ml concentration. The staining was developed with HRP-DAB detection method and the sections were counterstained using hematoxylin. This Survivin antibody generated an expected nuclear cytoplasmic immunostaining in a subset of cancer cells, which are potentially the proliferating cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Immunohistochemistry-Paraffin-NBP2-59503-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (1277A) [NBP2-59503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with Survivin Antibody (1277A) [NBP2-59503] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Immunocytochemistry-Immunofluorescence-NBP2-59503-img0005.jpg)
![Western Blot: Survivin Antibody (1277A) [NBP2-59503] - Total protein from human HeLa and A431 cell lines was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um Polyvinylidene fluoride (PVDF) membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml [NBP2-59503] in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note: predicted molecular weight is 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Western-Blot-NBP2-59503-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (1277A) [NBP2-59503] - An intracellular stain was performed on HeLa Cells with Survivin Antibody (1277A) [NBP2-59503] (blue) and a matched isotype control [MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody [F0111, R&D Systems].](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Flow-Intracellular-NBP2-59503-img0003.jpg)
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunocytochemistry-Immunofluorescence-NBP2-44687-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0004.jpg)
![Western Blot: TACC3 Antibody (OTI6F1) [NBP2-02619] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Western-Blot-NBP2-02619-img0010.jpg "Western Blot: TACC3 Antibody (OTI6F1) [NBP2-02619] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 .](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunocytochemistry-Immunofluorescence-NBP2-02619-img0011.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human prostate tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0009.jpg)
![Flow Cytometry: TACC3 Antibody (OTI6F1) [NBP2-02619] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Flow-Cytometry-NBP2-02619-img0001.jpg)
![Western Blot: TACC3 Antibody (OTI6F1) [NBP2-02619] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Western-Blot-NBP2-02619-img0002.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0003.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human colon tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0004.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human Kidney tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0005.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human liver tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0006.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human lung tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0007.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (OTI6F1) [NBP2-02619] - Staining of paraffin-embedded Human pancreas tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3-Antibody-OTI6F1-Immunohistochemistry-Paraffin-NBP2-02619-img0008.jpg)
![Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Western-Blot-NBP2-46199-img0006.jpg "Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.")
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0005.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0001.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0002.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0003.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0004.jpg)