| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87195-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-92717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73786 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47893-0.1mg | |||
| IHC-Prediluted | NBP2-48117 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-439SS | |||
| Azide and BSA Free | NBP2-80945 | |||
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87195 (A) shows similar protein distribution across tissues to independent antibody NBP1-87196 (B).](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0022.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87195 (A) shows similar protein distribution across tissues to independent antibody NBP1-87196 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCM1 Antibody [NBP1-87195] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87195-img0008.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0013.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0014.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0015.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0016.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human liver.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0018.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Staining of human ovary.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87195-img0020.jpg)
![Flow Cytometry: PCM1 Antibody [NBP1-87195] - Cardiomyocyte-specific Cre-mediated recombination.(A) Representative flow cytometry plots depicting the gating strategy for sorting of cardiomyocyte nuclei. Positive 7-AAD (left panel) & PCM-1 (middle panel) staining identified cardiomyocyte nuclei. Purity of sorted cardiomyocyte nuclei is demonstrated in the right panel. FSC represents forward scatter. (B) Recombination rates in sorted male cardiomyocyte nuclei. Recombination rates were calculated from relative expression of respective Dnmt3 exons compared to Sry levels (n = 6). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0131019), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87195_rabbit-polyclonal-pcm1-antibody-310202415395961.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90103-img0009.jpg "Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0014.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0011.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0012.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0013.jpg)
![Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Western-Blot-NB110-92717-img0002.jpg "Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Ser20] Antibody [NB110-92717] - Survivin [p Ser20] Antibody (NB110-92717) was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Immunocytochemistry-Immunofluorescence-NB110-92717-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89951-img0010.jpg "Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0015.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human duodenum shows strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0012.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human lymphoid tissues shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0013.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416113528.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416121943.jpg)
![Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Western-Blot-NBP2-37626-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunocytochemistry-Immunofluorescence-NBP2-37626-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-Paraffin-NBP2-37626-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-NBP2-37626-img0004.jpg)
![ELISA: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-ELISA-NBP2-37626-img0001.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0023.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube, liver, ovary and testis using Anti-PCM1 antibody NBP1-87196 (A) shows similar protein distribution across tissues to independent antibody NBP1-87195 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCM1 Antibody [NBP1-87196] - Staining of human cell line U-251 MG shows localization to nuclear membrane & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87196-img0018.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0015.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human ovary.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0021.jpg)
![Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87196-img0022.jpg)
![Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.](http://images.novusbio.com/fullsize/FoxM1-Antibody-Western-Blot-NBP1-30961-img0013.jpg "Western Blot: FoxM1 Antibody [NBP1-30961] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody [C3], C-term diluted at 1:5000.")
![Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-30961] - Representative images of immunoreactivity of nuclear FOXM1 in (I) benign cystadenoma, (II) borderline tumour, (III) stage I invasive cancer, (IV) stage II invasive cancer, (V) stage III invasive cancer and (VI) stage IV invasive cancer. Magnifications X400. Insets: Regions with higher magnifications of nuclear FOXM1 staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0113478), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FoxM1-Antibody-Immunohistochemistry-Paraffin-NBP1-30961-img0014.jpg)
![Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Western-Blot-NBP2-03644-img0002.jpg "Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NBP2-03644-img0014.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0013.jpg)
![Flow Cytometry: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Flow-Cytometry-NBP2-03644-img0001.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0006.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0007.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0009.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0011.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0005.jpg "Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TPX2 Antibody [NB500-184] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-184. TPX2 was also immunoprecipitated using NB500-183 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels. TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels Depletion of TPX2 by siRNA causes a decrease in H4K16ac levels whereas the levels of H3K9ac and H3K56ac remain unchanged. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Knockdown-Validated-NB500-184-img0004.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416182636.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416161844.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416171433.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416173534.jpg)
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunocytochemistry-Immunofluorescence-NBP2-44687-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0004.jpg)
![Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-1B12-Western-Blot-NB100-439-img0002.jpg "Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.")