| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11592 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04117-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02957-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP3-49541AFP405 | |||
| Alexa Fluor Plus 488 | NBP3-49541AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49541AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49541AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49541AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49541AFP680 | |||
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-PN-15-Immunohistochemistry-Paraffin-NBP2-45238-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).")
![Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04117-img0003.jpg "Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric cancer using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0002.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0001.jpg)
![Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/Smad2-Antibody-Western-Blot-NBP3-02957-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HRAS-Antibody-4Q7M5-Western-Blot-NBP3-15818-img0002.jpg "Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818] - Immunohistochemistry of paraffin-embedded human breast cancer using HRAS antibody (NBP3-15818) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HRAS-Antibody-4Q7M5-Immunohistochemistry-Paraffin-NBP3-15818-img0001.jpg)
![Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-278202415295577.jpg "Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HRAS Antibody (JE42-15) [NBP3-32413] - Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-298202414161896.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Immunohistochemical staining of formalin fixed and paraffin embedded human liver tissue sections using Clone RM486) at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp3-23472_rabbit-carbonic-anhydrase-ix-ca9-mab-24620241332466.png "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Immunohistochemical staining of formalin fixed and paraffin embedded human liver tissue sections using Clone RM486) at 1:100 dilution.")
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Western Blot of SW620 cell lysate using Clone RM486) at 1:2500 dilution.](http://images.novusbio.com/fullsize/nbp3-23472_rabbit-carbonic-anhydrase-ix-ca9-mab-22620242311011.png)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (RM486) [NBP3-23472] - Immunohistochemical staining of formalin fixed and paraffin embedded human stomach tissue sections using RM486 at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp3-23472_rabbit-carbonic-anhydrase-ix-ca9-mab-immunohistochemistry-paraffin-18920252341544.png)