| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12880-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88146-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47597-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12878-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97502-0.2mg | |||
| BSA Free | NBP1-97502SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44974 | |||
| Azide and BSA Free | NBP2-34751-0.1mg | |||
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Western-Blot-NBP2-12880-img0010.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0007.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0010.jpg "Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.")
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0001.jpg)
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0003.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0005.jpg)
![Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4 Analysis of TOMM22 expression in NIH/3T3.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Western-Blot-H00056993-M01-img0016.jpg "Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4 Analysis of TOMM22 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of monoclonal antibody to TOMM22 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00056993-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Immunohistochemistry-Paraffin-H00056993-M01-img0011.jpg)
![Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi ( Cat # H00056993-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody (M01), clone 4G4 (Cat # H00056993-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Western-Blot-H00056993-M01-img0012.jpg)
![Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4. Analysis of TOMM22 expression in PC-12.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Western-Blot-H00056993-M01-img0014.jpg)
![Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4. Analysis of TOMM22 expression in Raw 264.7.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Western-Blot-H00056993-M01-img0015.jpg)
![Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody (M01), clone 4G4.Lane 1: TOMM22 transfected lysate(15.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Western-Blot-H00056993-M01-img0017.jpg)
![Sandwich ELISA: TOMM22 Antibody (4G4) [H00056993-M01] - Detection limit for recombinant GST tagged TOMM22 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TOMM22-Antibody-4G4-Sandwich-ELISA-H00056993-M01-img0013.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0011.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32118-img0002.jpg)
![Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse lung. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-NBP1-32118-img0007.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Sample(30 ug of whole cell lysate)A:293TB:A431 7. 5% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/GRP94-Antibody-Western-Blot-NBP1-32118-img0005.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0003.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse skin. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0006.jpg)
![IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.Sample: Paraformaldehyde-fixed zebrafish embryos.Hsp90 beta antibody dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-immunohistochemistry-whole-mount-3011202314722..jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-32118 diluted at 1:10000](http://images.novusbio.com/fullsize/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-910202420131243.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88146 (A) shows similar protein distribution across tissues to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0014.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88146 (A) shows similar protein distribution across tissues to independent antibody NBP1-88147 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining in human testis and skeletal muscle tissues using anti-CCT2 antibody. Corresponding CCT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0012.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0011.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0010.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human tonsil.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0017.jpg)
![Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-88146-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TCP1-beta Antibody [NBP1-88146] - Analysis using Anti-CCT2 antibody NBP1-88146 (A) shows similar pattern to independent antibody NBP1-88147 (B).](http://images.novusbio.com/fullsize/TCP1-beta-Antibody-Western-Blot-NBP1-88146-img0015.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-6520251624419.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-88146_rabbit-polyclonal-tcp1-beta-antibody-65202516252810.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP2-47597] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.](http://images.novusbio.com/fullsize/TRAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47597-img0004.jpg "Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP2-47597] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.")
![Western Blot: TRAP1 Antibody [NBP2-47597] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/antibody/nbp2-47597_rabbit-polyclonal-trap1-antibody-western-blot-12320248571..jpg)
![Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Western-Blot-NBP2-12878-img0011.jpg "Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunocytochemistry-Immunofluorescence-NBP2-12878-img0007.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0014.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0012.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0013.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Western-Blot-NBP1-97502-img0002.jpg "Western Blot: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.")
![Immunohistochemistry-Paraffin: Calreticulin Antibody (FMC 75) [NBP1-97502] - Analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10ug/ml.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-FMC-75-Immunohistochemistry-Paraffin-NBP1-97502-img0001.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0022.jpg "Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78614-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Paraffin-embedded mouse lymph node. ERp57 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0021.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0013.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NB100-78614-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: PDIA3 protein stained by ERp57 antibody, C-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78614-img0011.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - H1299 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0012.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Mouse intestine. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0016.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Rat middle brain. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NB100-78614-img0017.jpg)
![Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Western-Blot-NBP2-32865-img0005.jpg "Western Blot: HSP27 Antibody (SPM252) [NBP2-32865] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (SPM252).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (SPM252) [NBP2-32865] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunocytochemistry-Immunofluorescence-NBP2-32865-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Flow-Cytometry-NBP2-32865-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))](http://images.novusbio.com/fullsize/HSP27-Antibody-SPM252-Immunohistochemistry-Paraffin-NBP2-32865-img0001.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)