| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25154SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90291-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84797-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71488 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70954 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61832-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0007.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.")
![Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Western-Blot-NBP2-25154-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90291-img0004.jpg "Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0009.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0013.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0014.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0011.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-47477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47477-img0004.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0010.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0011.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84797 (A) shows similar protein distribution across tissues to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis using Anti-PDIA3 antibody NBP1-84797 (A) shows similar pattern to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84797-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84797] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84797-img0007.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0020.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0021.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Western-Blot-NBP2-01652-img0009.jpg "Western Blot: ERp72 Antibody (2B9) [NBP2-01652] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.")
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human tonsil using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0008.jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Western-Blot-NBP2-01652-img0001.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0002.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0003.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0004.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0005.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human lymphoma tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0006.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human pancreas tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0007.jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - Western Blot of lysate of PC-3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-01652_mouse-erp72-mab-oti2b9-western-blot-672023153749..jpg)
![Immunocytochemistry/Immunofluorescence: Mouse ERp72 mAb (OTI2B9) [NBP2-01652] - Primary mouse hepatocytes stained with ERp72 mAb. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-01652_mouse-erp72-mab-oti2b9-immunocytochemistry-immunofluorescence-7122024113558..png)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Western-Blot-NBP2-02190-img0011.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP2-02190-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Flow-Cytometry-NBP2-02190-img0010.jpg)
![Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Western-Blot-NBP2-02190-img0009.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0006.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70954-img0002.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70954-img0004.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0009.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70954-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70954-img0001.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0005.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0006.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0007.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0008.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0010.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0009.jpg "Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0005.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0007.jpg)
![Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Flow-Cytometry-NBP2-61832-img0002.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0008.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0004.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0006.jpg)
![ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-ELISA-NBP2-61832-img0001.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - RanGTPase detected with Mouse Monoclonal Ran Antibody (8D1A6) in HEK293T and LNCaP C42. Lanes are labeled as follows: 1. Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4. LNCaP C42 5. LNCaP C42. Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with infrared scanner. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-61832_mouse-monoclonal-ran-antibody-8d1a6-western-blot-162023155342.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NB100-41377-img0006.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41377-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NB100-41377-img0008.jpg)