| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50051SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82249-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05552-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72710 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72643 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65362-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37736-0.025ml | |||
![Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of Tau (p Ser396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Tau (p Ser396) antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Western-Blot-NBP2-67702-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of Tau (p Ser396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Tau (p Ser396) antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Staining phospho -Tau(S396) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunocytochemistry-Immunofluorescence-NBP2-67702-img0004.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Tau [p Ser396] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0008.jpg)
![Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Western blot analysis of Tau on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Western-Blot-NBP2-67702-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Staining phospho -Tau(S396) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunocytochemistry-Immunofluorescence-NBP2-67702-img0001.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse brain tissue using anti- phospho -Tau(S396) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0002.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded rat brain tissue using anti- phospho -Tau(S396) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0003.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau [p Ser396] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0007.jpg)
![Western Blot: Tau Antibody (5B10) [NBP2-50051] - Analysis of tissue lysates using mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight big tau Isoforms.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Western-Blot-NBP2-50051-img0005.jpg "Western Blot: Tau Antibody (5B10) [NBP2-50051] - Analysis of tissue lysates using mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight big tau Isoforms.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green, and costained with chicken pAb to GFAP, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-50051 stains perikarya, dendrites and axons of neurons, while the GFAP antibody labels astrocytes.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Immunocytochemistry-Immunofluorescence-NBP2-50051-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - E18 rat brain cultures were grown for 10 days, fixed and immunostained with NBP2-50051 in red and chicken antibody to MAP2 (NB300-213, green) and for DNA (blue). NBP2-50051 stains the neuronal perikarya, dendrites and axonal process strongly and does not stain non neuronal cells in these cultures. The MAP2 antibody also stains neurons only, but only the perikarya and dendrites. As a result perikarya and dendrites appears yellow, since they contain both tau and MAP2, while axons appear red as they contain only tau.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Immunocytochemistry-Immunofluorescence-NBP2-50051-img0003.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg)
![Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74638_rabbit-polyclonal-astrin-antibody-31020241612667.jpg)
![Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Western-Blot-NB100-82249-img0002.jpg "Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.")
![Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr231) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr231]-Antibody-Immunohistochemistry-NB100-82249-img0001.jpg)
![Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-Western-Blot-NBP3-05552-img0002.jpg "Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NBP3-05552] - Immunofluorescent analysis of rat brain stem section stained with goat pAb to MAP2 at a dilution of 1:2,000 in red, and costained with mouse mAb to MBP at a dilution of 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. MAP2 Antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the MBP antibody stains myelin sheath around axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05552-img0001.jpg)
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)
![Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Western-Blot-NBP1-47833-img0001.jpg "Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1.")
![Immunocytochemistry/Immunofluorescence: MEK1 Antibody (1F5) [NBP1-47833] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK1.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunocytochemistry-Immunofluorescence-NBP1-47833-img0009.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded prostate tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0008.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0002.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0003.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded colon tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0004.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded endometrium tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0005.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Ovary tissue using anti-MEK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0006.jpg)
![Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded pancreas tissue using anti-MEK1mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0007.jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0006.jpg "Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.")
![Flow Cytometry: CD45 Antibody [NBP2-15811] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4 C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488- Conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0005.jpg)
