| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB400-140SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55918-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92925-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50194-0.025ml | |||
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0016.jpg "Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: ABCG1 Antibody - BSA Free [NB400-132] - Staining of human lung, alveolar macrophages.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-132-img0013.jpg)
![Flow (Intracellular): ABCG1 Antibody - BSA Free [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody.](http://images.novusbio.com/fullsize/ABCG1-Antibody-BSA-Free-Flow-Intracellular-NB400-132-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Cells were pretreated with 5 uM F12511 or 5 uM MG132 for 30 min and then exposed to 5 or 50 uM 24S-OHC for 6 h. Whole-cell lysates were immunoblotted with antibodies specific for ABCG1 or beta-actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31285856/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1 Antibody - BSA Free-Western Blot-NB400-132-img0017.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0005.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0009.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated macrophages, Lane 3: T0901713 treated macrophages, Lane 4: T091713 treated macrophages.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0004.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected subcutaneously with PBS or 0.8 ug/g LPS as indicated, followed 4 hr later by intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages. ABCG1 in liver lysates (40 ug protein) was identified by immunoblotting and quantified by densitometry. Results from each of the 3 experiments (n = 4-5 per group per experiment) were expressed relative to PBS-injected WT mice after normalization to beta-actin. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/283486/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0014.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1 protein stability. THP‐1 cells were differentiated as described in Figure 2. Then cells were loaded with unlabeled cholesterol & treated with 6‐dihydroparadol (10 μm), or solvent vehicle (0.1% DMSO, control) for 24 h. Cells were lysed at different time points (0, 1, 2, 3, 4, & 6 h) after treatment with the protein synthesis inhibitor cycloheximide (CHX, 100 μm). The protein levels of both ABCA1 & ABCG1 were detected by Western blot analysis. The data points represent mean ± SD from three independent experiments. *p < 0.05 & **p < 0.01 versus control at the same time point (determined by Student's t‐test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-132_rabbit-polyclonal-abcg1-antibody-310202415291917.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Evodiamine enhances apo A1-mediated ChE from THP-1 macrophages & increases ABCA1 protein level. (a) Differentiated THP-1 cells were loaded with [3H]-cholesterol together with the indicated treatments for 24 h. On the next day, the cells were washed twice with PBS & incubated with the same compounds [solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (1–20 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control] with or without 10 µg/mL apo A1. Extracellular as well as intracellular radioactivities were quantified with scintillation counter. Differentiated THP-1-derived macrophages were treated with solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (10 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control. After 24 h incubation, the cells were lysed & 20 μg protein was resolved via SDS-PAGE. Immunodetection was performed with antibodies against the indicated proteins, ABCA1 (b), ABCG1 (c), & SR-B1 (d), & visualized by chemiluminescence detection. All experiments were performed at least three times & data are presented as means ± S.D. vs. solvent vehicle control, *p < 0.05, **p < 0.01, ***p < 0.001, n.s. no significance (ANOVA/Bonferroni). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30038271), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-132_rabbit-polyclonal-abcg1-antibody-31020241618932.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0011.jpg "Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.")
![Immunocytochemistry/Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-140-img0013.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0005.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Analysis of Cyp-46 in HEK293 cells overexpressing CYP46A1 or transfected with an empty vector using NB400-140.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0010.jpg)
![Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Western-Blot-NB100-74457-img0004.jpg "Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74457-img0003.jpg)
![Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Western-Blot-H00007376-M04-img0008.jpg "Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of monoclonal antibody to NR1H2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00007376-M04-img0006.jpg)
![Western Blot: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Analysis of NR1H2 expression in IMR-32 (Cat # L008V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Western-Blot-H00007376-M04-img0009.jpg)
![Sandwich ELISA: LXR beta/NR1H2 Antibody (1E1) [H00007376-M04] - Detection limit for recombinant GST tagged NR1H2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1E1-Sandwich-ELISA-H00007376-M04-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1464-img0002.jpg "Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Flow-Cytometry-NB100-1464-img0001.jpg)
![Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-91020242014662.jpg "Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202420104468.jpg)
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202419593547.jpg)
![Western Blot: ABCG1 Antibody [NBP2-54682] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Western-Blot-NBP2-54682-img0003.jpg "Western Blot: ABCG1 Antibody [NBP2-54682] - Analysis in human cell line Daudi.")
![Immunohistochemistry-Paraffin: ABCG1 Antibody [NBP2-54682] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Immunohistochemistry-Paraffin-NBP2-54682-img0001.jpg)
![Western Blot: LXR beta/NR1H2 Antibody [NBP2-55918] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Western-Blot-NBP2-55918-img0002.jpg "Western Blot: LXR beta/NR1H2 Antibody [NBP2-55918] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NBP2-55918] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55918-img0001.jpg)
![Western Blot: CYP46A1 Antibody [NBP2-92925] - Analysis of extracts of various cell lines, using CYP46A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NBP2-92925-img0002.jpg "Western Blot: CYP46A1 Antibody [NBP2-92925] - Analysis of extracts of various cell lines, using CYP46A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-N8-P6E4-H8-Western-Blot-NBP2-50194-img0002.jpg "Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.")
![Immunohistochemistry: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - Shows strong cytoplasmic staining in metastatic colorectal cancer (A) and primary colorectal (B) compared to normal colon mucosa (C).](http://images.novusbio.com/fullsize/CYP46A1-Antibody-N8-P6E4-H8-Immunohistochemistry-NBP2-50194-img0001.jpg)
![Western Blot: LXR beta/NR1H2 Antibody (1B6) [H00007376-M03] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1B6-Western-Blot-H00007376-M03-img0003.jpg "Western Blot: LXR beta/NR1H2 Antibody (1B6) [H00007376-M03] - Analysis of NR1H2 expression in PC-12 (Cat # L012V1).")
![Western Blot: LXR beta/NR1H2 Antibody (1B6) [H00007376-M03] - Analysis of NR1H2 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/LXR-beta-NR1H2-Antibody-1B6-Western-Blot-H00007376-M03-img0004.jpg)