| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91659-25ul | |||
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0010.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Flow-Cytometry-NBP2-67295-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0011.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minut](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0012.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0013.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] -Analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody at 1/5,000 dilution.Lane 1: Saos-2 cell lysate (15ug/Lane)Lane 2: HeLa cell lysate (15 ug/Lane)Lane 3: A549 cell lysate (15 ug/Lane)Lane 4: Mouse liver tissue lysate (20 ug/Lane)Lane 5: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 57 kDaObserved band size: 75 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67295_rabbit-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-sa40-00-western-blot-1482024111635..jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249342724.jpg "Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249422658.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413360.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249383187.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249362716.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413379.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249403843.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP1-84687-img0005.jpg "Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0020.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0012.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0013.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP1-84687 (A) shows similar protein distribution across tissues to independent antibody NBP2-38622 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP1-84687 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0018.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human pancreas shows moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0019.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0021.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91660-img0004.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human pancreas shows no postivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0014.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249374918.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249383191.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-212024940384.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249393565.jpg)
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-immunohistochemistry-paraffin-2611202518413916.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-western-blot-26112025198367.jpg)
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-immunohistochemistry-paraffin-261120251838151.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241943225.jpg "Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunofluorescence of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241928876.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Western blot analysis of Calcium-sensing R/CaSR in EL4 cell lysate with Calcium-sensing R/CaSR antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120242092044.gif)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Western-Blot-NBP3-19565-img0003.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunocytochemistry-Immunofluorescence-NBP3-19565-img0001.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunohistochemistry of Alkaline Phosphatase, Tissue Non-Specific in paraffin-embedded Human lung cancer tissue using NBP3-19565 at dilution 1/50](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunohistochemistry-Paraffin-NBP3-19565-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19565_rb-alkaline-phosphatase-tissue-non-specific-mab-s08-6h0-11320251555912.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27291-img0002.jpg "Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human pancreas shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0014.jpg)