| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74051 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47397-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35155-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06239-50ul | |||
![Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - NR and Metf promote FoxO1-mediated Lipa upregulation in visceral AT of adult mice. Adult C57/BL6 mice (5 months) were nutrient restricted (NR) by 24 h fasting or treated for 10 days with Metf (400 mg/kg) dissolved in drinking water (n=4 mice per group). Western blot of FoxO1 and Lipa in total protein extracts of explanted visceral (epididymal) AT. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013404) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0009.jpg)
![Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Simple-Western-NBP1-54155-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody NBP1-54155 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54155-img0010.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free [NBP1-54155] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (NBP1-54155). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lipase-A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-54155-img0011.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0001.jpg)
![Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP1-54155-img0005.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416154610.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416165629.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - AMPK drives Lipa-released FFAs oxidation restraining energetic catastrophe. (a) 3T3-L1 cells were transfected with DN-AMPK or empty vector. RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha , carnitine palmitoyltransferase 1b & acyl-CoA oxidase 1 mRNA levels were performed after 4 h of NR or 16 h of Metf treatment. Dashed line indicates the mRNA value of untreated DN-AMPK cells (Ctr). mRNA levels of untreated cells transfected with empty vector were similar to untreated DN-AMPK cells (data not shown). (b) Cheminoluminescent assay of ATP level in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector after 8 h NR or 16 h Metf treatment. ATP level was expressed as pmol ATP per mg protein. (c) After 8 h of NR or 16 h Metf treatment, FFAs were enzymatically detected in culture medium of 3T3-L1 adipocytes transfected with DN-AMPK or empty vector. Values were expressed as μg FFAs per mg protein. (d) Left panel: western blot of AMPKpT172, PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 8 h NR. Right panel: cytofluorimetric analysis of apoptosis in DN-AMPK cells subjected to 8 h NR. (e) Western blot of PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & treated with Metf for 16 h. (f) Western blot of FoxO1, Lipa, LC3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 4 h NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05, °°P<0.01 versus Metf treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241618936.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171493.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171436.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - Lipa downregulation impairs lipid breakdown & elicits cell death in nutrient restricted adipocytes. (a) 3T3-L1 adipocytes were transfected with siRNA against Lipa (Lipa(−)) or with a scramble siRNA (Scr). Western blot of Lipa, PARP-1 & cleaved form of caspase-3 in total protein extracts from 3T3-L1 adipocytes after 4 h of NR. (b) TG content was quantified by ORO staining in fixed 3T3-L1 adipocytes 6 h after NR. (c) RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha & carnitine palmitoyltransferase 1b mRNA levels was performed in 3T3-L1 adipocytes 4 h after NR. (d) FFAs were analyzed in culture medium 6 h after NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05 versus NR treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416163713.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241616083.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0015.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0012.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0013.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0014.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0005.jpg "Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)")
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (1F6) [NBP2-03323] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunocytochemistry-Immunofluorescence-NBP2-03323-img0010.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0009.jpg)
![Flow Cytometry: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Flow-Cytometry-NBP2-03323-img0001.jpg)
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0003.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0004.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0006.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0007.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0008.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0002.jpg "Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71363-img0004.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0010.jpg)
![Flow Cytometry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71363-img0003.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0001.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0005.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0006.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0007.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0008.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0009.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0011.jpg)
![Western Blot: GALE Antibody (1C4) [NBP2-03390] Analysis of extracts (35ug) from 9 different cell lines by using anti-GALE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Western-Blot-NBP2-03390-img0010.jpg "Western Blot: GALE Antibody (1C4) [NBP2-03390] Analysis of extracts (35ug) from 9 different cell lines by using anti-GALE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human prostate tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0009.jpg)
![Western Blot: GALE Antibody (1C4) [NBP2-03390] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALE.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Western-Blot-NBP2-03390-img0001.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0002.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0003.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0004.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0005.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human Kidney tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0006.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human liver tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0007.jpg)
![Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human lymph node tissue using anti-GALE mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GALE-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-03390-img0008.jpg)
![GALE Antibody (OTI1C4)[NBP2-03390]Simple Western� analysis of endogenous protein SAMHD1 from THP-1 lysates (0.1 mg/mL) using SAMHD1 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-03390_mouse-gale-mab-oti1c4-simple-western-131120251236351.jpg%253fstore=america&image-type=image)
![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0006.jpg "Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunocytochemistry-Immunofluorescence-NBP2-01346-img0005.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Human prostate tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0004.jpg)
![Flow Cytometry: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Flow-Cytometry-NBP2-01346-img0001.jpg)
![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0002.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Analysis in human spleen and skeletal muscle tissues. Corresponding Lipase A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Analysis in human spleen and skeletal muscle tissues. Corresponding Lipase A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP2-47397] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP2-47397-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP2-47397] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47397-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0015.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0008.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0009.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP2-47397-img0011.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in a subset of non-germinal center cells.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP2-47397-img0012.jpg)
![Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NB100-1189-img0006.jpg "Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189] - IHC staining of CLPP in paraffin embedded Human Liver using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NB100-1189-img0004.jpg)
![Western Blot: CLPP Antibody [NB100-1189] - Analysis of CLPP in human muscle lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 1 ug/ml. Primary antibody incubated for 1 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NB100-1189-img0005.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415133421.jpg "Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415121312.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202414201353.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody [NBP3-35155] - Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415175267.jpg)
![Western Blot: SEC14L2 Antibody [NBP3-35155] - Western blot analysis of various lysates using SEC14L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415152860.jpg)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human liver with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251433153.png)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human pancreas with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-2352025112932.png)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06239 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251633504.png)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP2-19360-img0002.jpg "Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Lipase A Antibody [NBP2-19360] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lipase A antibody [N3C3] (NBP2-19360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-910202420131257.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-19360] -Mouse kidney.LAL stained by LAL antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-immunohistochemistry-paraffin-11112024104132..png)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (NBP2-19360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-western-blot-261120251847578.jpg)
![LAL antibody [N3C3] detects LAL protein at lysosome by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: LAL stained by LAL antibody [N3C3] (NBP2-19360) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19360_rabbit-polyclonal-lipase-a-antibody-immunocytochemistry-immunofluorescence-261120251832194.jpg)