| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72550 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-115SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-166 | |||
| BSA Free | NB400-166SS | |||
| BSA Free | NB400-163SS | |||
| Azide and BSA Free | NBP2-80557 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB3664-SP | |||
![Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NB110-57150-img0003.jpg "Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.")
![Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Immunohistochemistry-NB110-57150-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16400-img0002.jpg "Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0011.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0001.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0007.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] diluted at 1:500](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0010.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251827016.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251823622.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518204710.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518422918.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Western-Blot-NBP2-46220-img0017.jpg "Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.")
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0016.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0001.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0002.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0003.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0005.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0007.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0008.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0009.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0010.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0011.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0012.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0013.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0014.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-1A5-Immunohistochemistry-NBP2-46220-img0015.jpg)
![Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FACL4-Antibody-3I8D10-Immunoprecipitation-NBP3-16012-img0002.jpg "Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.")
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-5220251718945.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184534.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517202918.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184563.jpg)
![Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172885.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213510.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213524.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172887.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Knockdown-Validated-NB400-115-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NB400-115-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-115-img0005.jpg)
![Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Simple-Western-NB400-115-img0006.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0010.jpg "Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0008.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0007.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Detection of ABCA7 using NB 400-163 at various dilutions in ABCA7 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0006.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using NB 400-163 at a 1:2,000 dilution.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0005.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Western-Blot-MAB3664-img0002.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed HepG2 cell using ApoA1 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Immunocytochemistry-MAB3664-img0001.jpg)
![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using ApoA1 Monoclonal Antibody at 1 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Immunohistochemistry-MAB3664-img0003.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-I/ApoA1 Antibody (2083D) MAB3664AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Flow-Cytometry-MAB3664-img0006.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB3664, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-2083D-Flow-Cytometry-MAB3664-img0005.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunocytochemistry-Immunofluorescence-NBP3-13666-img0002.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunohistochemistry-Paraffin-NBP3-13666-img0003.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0004.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [HL44] (NBP3-13666) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202419593532.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202420122075.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-1610202419414568.jpg)
![Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB100-57850-img0008.jpg "Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57850-img0006.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NB100-57850-img0002.jpg)
![Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB100-57850-img0005.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NB100-57850-img0001.jpg)
![Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of extracts of various cell lines, using Fatty Acid Synthase/FASN antibody (NBP3-15634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Western-Blot-NBP3-15634-img0004.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of extracts of various cell lines, using Fatty Acid Synthase/FASN antibody (NBP3-15634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of 300ug extracts of HeLa cells using 3ug Fatty Acid Synthase/FASN antibody (NBP3-15634). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase/FASN antibody (NBP3-15634) at a dilition of 1:500.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunoprecipitation-NBP3-15634-img0005.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody (4U9S3) [Fatty Acid Synthase/FASN] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Fatty Acid Synthase/FASN Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15634_rabbit-fatty-acid-synthase-fasn-mab-4u9s3-5220251731557.jpg)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of NIH-3T3 cells using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-4U9S3-Immunocytochemistry-Immunofluorescence-NBP3-15634-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [Fatty Acid Synthase/FASN] - Immunofluorescence analysis of HeLa cells using Fatty Acid Synthase/FASN Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15634_rabbit-fatty-acid-synthase-fasn-mab-4u9s3-5220251734578.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody (4U9S3) [Fatty Acid Synthase/FASN] - Immunohistochemistry analysis of paraffin-embedded Mouse skin tissue using Fatty Acid Synthase/FASN Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15634_rabbit-fatty-acid-synthase-fasn-mab-4u9s3-52202517352796.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody (4U9S3) [Fatty Acid Synthase/FASN] - Immunohistochemistry analysis of paraffin-embedded Rat fat tissue using Fatty Acid Synthase/FASN Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15634_rabbit-fatty-acid-synthase-fasn-mab-4u9s3-52202517311054.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody (4U9S3) [Fatty Acid Synthase/FASN] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Fatty Acid Synthase/FASN Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15634_rabbit-fatty-acid-synthase-fasn-mab-4u9s3-52202517361226.jpg)
![Western Blot: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Western-Blot-NBP2-67414-img0008.jpg "Western Blot: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunocytochemistry-Immunofluorescence-NBP2-67414-img0004.jpg)
![Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human lung tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunohistochemistry-Paraffin-NBP2-67414-img0007.jpg)
![Flow Cytometry: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Flow-Cytometry-NBP2-67414-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunocytochemistry-Immunofluorescence-NBP2-67414-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunocytochemistry-Immunofluorescence-NBP2-67414-img0003.jpg)
![Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunohistochemistry-Paraffin-NBP2-67414-img0005.jpg)
![Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LDLR-Antibody-SJ0197-Immunohistochemistry-Paraffin-NBP2-67414-img0006.jpg)