| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71189 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74505 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-94162SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73852 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-99585SS | |||
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0022.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.")
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded prostate tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0020.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Leukotriene A4 hydrolase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Leukotriene A4 hydrolase.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0021.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0013.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0014.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded endometrium tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0018.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0017.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded pancreas tissue using anti-Leukotriene A4 hydrolasemouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0019.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunoprecipitation-NBP1-47829-img0023.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunocytochemistry-Immunofluorescence-NB100-74398-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing staining in the cytoplasm and membrane of rat liver tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the membrane of Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-1A2-Antibody-MC1-Immunohistochemistry-Paraffin-NB100-74398-img0007.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Western-Blot-NBP2-02710-img0008.jpg "Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunocytochemistry-Immunofluorescence-NBP2-02710-img0007.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-02710-img0006.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Thromboxane synthase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0003.jpg)
![Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Thromboxane synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Thromboxane synthase.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Western-Blot-NBP2-02710-img0004.jpg)
![Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human Kidney tissue using anti-Thromboxane synthase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-02710-img0005.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Analysis of Hela cells, using anti-Thromboxane synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0001.jpg)
![Flow Cytometry: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Analysis of Jurkat cells, using anti-Thromboxane synthase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Thromboxane-synthase-Antibody-2C1-Flow-Cytometry-NBP2-02710-img0002.jpg)
![Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.](http://images.novusbio.com/fullsize/MAT1-2A-Antibody-Western-Blot-NB110-94162-img0003.jpg "Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.")
![Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MAT1-2A-Antibody-Simple-Western-NB110-94162-img0004.jpg)
![Western Blot: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Retinol Binding Protein RBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Retinol Binding Protein RBP.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Western-Blot-NBP2-00637-img0003.jpg "Western Blot: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Retinol Binding Protein RBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Retinol Binding Protein RBP.")
![Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Retinol Binding Protein RBP.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP2-00637-img0009.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Staining of paraffin-embedded Human Ovary tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunohistochemistry-Paraffin-NBP2-00637-img0008.jpg)
![Flow Cytometry: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Retinol Binding Protein RBP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Flow-Cytometry-NBP2-00637-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Immunofluorescent staining of HeLa cells using anti-Retinol Binding Protein RBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP2-00637-img0004.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunohistochemistry-Paraffin-NBP2-00637-img0005.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Staining of paraffin-embedded Human endometrium tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunohistochemistry-Paraffin-NBP2-00637-img0006.jpg)
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Staining of paraffin-embedded Human liver tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Immunohistochemistry-Paraffin-NBP2-00637-img0007.jpg)
![Flow Cytometry: Retinol Binding Protein RBP Antibody (2H3) [NBP2-00637] - Analysis of Hela cells, using anti-Retinol Binding Protein RBP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-2H3-Flow-Cytometry-NBP2-00637-img0001.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Western-Blot-NBP2-75461-img0008.jpg "Western Blot: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of Cytochrome P450 17A1 on rat testis tissue lysates with Rabbit anti-Cytochrome P450 17A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0007.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of SH-SY-5Y cells with Cytochrome P450 17A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Flow-Cytometry-NBP2-75461-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunocytochemistry-Immunofluorescence-NBP2-75461-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human breast tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (JB93-32) [NBP2-75461] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 17A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytochrome-P450-17A1-Antibody-JB93-32-Immunohistochemistry-Paraffin-NBP2-75461-img0006.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBS Antibody (519) [NBP2-43604] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBS Antibody (519) [NBP2-43604] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (519) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0008.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded mouse kidney. CBS antibody [519] diluted at 1:250.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Immunohistochemistry-Paraffin-NBP2-43604-img0006.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 30 ug Raji whole cell lysate/extract B. 30 ug K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0001.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:2000.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0002.jpg)
![Western Blot: CBS Antibody (519) [NBP2-43604] - Analysis of A. 50 ug rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [519] dilution: 1:1000](http://images.novusbio.com/fullsize/CBS-Antibody-519-Western-Blot-NBP2-43604-img0003.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody (519) [NBP2-43604] - Paraffin-embedded rat liver. CBS antibody [519] diluted at 1:250.](http://images.novusbio.com/fullsize/CBS-Antibody-519-Immunohistochemistry-Paraffin-NBP2-43604-img0005.jpg)
![Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43604_mouse-monoclonal-cbs-antibody-519-western-blot-2611202518425420.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (NBP2-43604) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43604_mouse-monoclonal-cbs-antibody-519-western-blot-261120251832450.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunocytochemistry-Immunofluorescence-NBP2-43572-img0009.jpg "Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IDH2 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0003.jpg)
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0004.jpg)
![Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Western-Blot-NBP2-43572-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunocytochemistry-Immunofluorescence-NBP2-43572-img0001.jpg)
![Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/IDH2-Antibody-673-Immunohistochemistry-Paraffin-NBP2-43572-img0012.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-western-blot-261120251825320.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-western-blot-2611202518255020.jpg)
![IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IDH2 antibody [GT673] (NBP2-43572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43572_mouse-monoclonal-idh2-antibody-673-immunohistochemistry-paraffin-2611202518451323.jpg)
![Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NB110-99585-img0002.jpg "Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-99585-img0004.jpg)
![Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Simple-Western-NB110-99585-img0005.jpg)