| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| BSA Free | NBP3-07012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22356SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06277SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90071-25ul | |||
![Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Western-Blot-NBP2-67766-img0007.jpg "Western Blot: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HeLa cell lysateLane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysateLane 5: human lung carcinoma tissue lysate.")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (SD20-92) [NBP2-67766] - Staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunocytochemistry-Immunofluorescence-NBP2-67766-img0005.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded mouse colon tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0017.jpg)
![Flow Cytometry: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Flow-Cytometry-NBP2-67766-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0008.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human prostate carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0009.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human skin tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0010.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0011.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human stomach carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0012.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0014.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human thyroid tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0015.jpg)
![Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human small intestine tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ATF4-Antibody-SD20-92-Immunohistochemistry-Paraffin-NBP2-67766-img0016.jpg)
![Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Western-Blot-NBP2-33250-img0004.jpg "Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33250-img0002.jpg)
![Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33250-img0003.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0007.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0006.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0012.jpg "Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0011.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0005.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0007.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0008.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse ovary. Aconitase 2 antibody [N1N3] dilution: 1:500](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0010.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245] - Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [N1N3] . Western blot analysis was performed using Aconitase 2 antibody [N1N3] .EasyBlot anti-Rabbit IgG was used as a secondary reagent](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP2-15245-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (NBP2-15245) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-910202420134052.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP2-15245 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-9102024205635.jpg)
![Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (NBP2-15245) diluted at 1:1000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-immunocytochemistry-immunofluorescence-2611202518384020.jpg)
![Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.](http://images.novusbio.com/fullsize/Torc2-Antibody-Western-Blot-NBP2-22356-img0003.jpg "Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.")
![Immunocytochemistry/Immunofluorescence: TORC2 Antibody [NBP2-22356] - Torc2 Antibody [NBP2-22356] - Torc2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Torc2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22356-img0002.jpg)
![Immunohistochemistry: Torc2 Antibody [NBP2-22356] - IHC staining of Torc2 in mouse thymus.](http://images.novusbio.com/fullsize/Torc2-Antibody-Immunohistochemistry-NBP2-22356-img0005.jpg)
![Simple Western: TORC2 Antibody [NBP2-22356] - Lane view shows a specific band for TORC1 at a dilution of 1:100 in Jurkat cell lysate at 200 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 94 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/TORC2-Antibody-Simple-Western-NBP2-22356-img0007.jpg)
![Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Western-Blot-H00000468-M01-img0009.jpg "Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of monoclonal antibody to ATF4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00000468-M01-img0007.jpg)
![Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Western Blot analysis of ATF4 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Western-Blot-H00000468-M01-img0001.jpg)
![ELISA: ATF4 Antibody (2B3) [H00000468-M01] - Detection limit for recombinant GST tagged ATF4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-ELISA-H00000468-M01-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0015.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Western blot analysis of Glucosylceramidase/GBA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0001.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0003.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0014.jpg)
![Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB600-973-img0001.jpg "Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.")
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NB600-973] - LRRK2 accumulates in globules in alphaS tg mice. Double immunofluorescence for alphaS with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alphaS tg mice. Scale bar = 5 um for all panels. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-973-img0003.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NB600-973] - Staining in human liver tissue using a 1:200 dilution.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NB600-973-img0002.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg "Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+Â UPN7), biphasic (BIP BAP1-Â UPN16) and sarcomatous (BAP1+Â UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg)
![Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (NBP2-16749) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-immunocytochemistry-immunofluorescence-261120251823305.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody [NBP1-90071] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-90071-img0017.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody [NBP1-90071] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-90071-img0021.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody [NBP1-90071] - Staining of human duodenum shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-90071-img0019.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody [NBP1-90071] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-90071-img0020.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody [NBP1-90071] - Staining of pig aortic valve tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-90071-img0013.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Immunohistochemistry-Paraffin-NBP1-32271-img0008.jpg "Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Western-Blot-NBP1-32271-img0013.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-91020242013402.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-1610202419414552.jpg)
![Glucosylceramidase/GBA antibody [C1C3] detects Glucosylceramidase/GBA protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glucosylceramidase/GBA stained by Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-immunocytochemistry-immunofluorescence-141120251833710.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-western-blot-1411202518431318.jpg)