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![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Western-Blot-NBP1-22974-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope")
![Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (A8) [NBP3-42169] - DAB staining on IHC-P; Sample: Porcine Small intestine Tissue; Primary Ab: 30ug/ml NBP3-42169; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42169_mouse-apolipoprotein-a-iv-apoa4-mab-a8-612202421121312.jpg "Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (A8) [NBP3-42169] - DAB staining on IHC-P; Sample: Porcine Small intestine Tissue; Primary Ab: 30ug/ml NBP3-42169; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (A8) [NBP3-42169] - Sample: Porcine Small intestine lysate Primary Ab: 3ug/ml NBP3-42169; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42169_mouse-apolipoprotein-a-iv-apoa4-mab-a8-612202421373069.jpg)
![Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (A8) [NBP3-42169] - Sample: Human Serum Primary Ab: 3ug/ml NBP3-42169; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42169_mouse-apolipoprotein-a-iv-apoa4-mab-a8-61220242117599.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20472-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Sphingosine Kinase 1/SPHK1 antibody (NBP2-20472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20472_rabbit-polyclonal-sphingosine-kinase-1-sphk1-antibody-9102024205622.jpg)
![Immunohistochemistry-Paraffin: CFTR Antibody (SPM176) [NBP2-53373] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (SPM176).](http://images.novusbio.com/fullsize/CFTR-Antibody-SPM176-Immunohistochemistry-Paraffin-NBP2-53373-img0001.jpg "Immunohistochemistry-Paraffin: CFTR Antibody (SPM176) [NBP2-53373] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (SPM176).")
![Western Blot: thioredoxin reductase 1 Antibody [NB300-879] - (0.1ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/thioredoxin-reductase-1-Antibody-Western-Blot-NB300-879-img0002.jpg "Western Blot: thioredoxin reductase 1 Antibody [NB300-879] - (0.1ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-06019] - Staining of Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-A-IV-ApoA4-Antibody-Immunohistochemistry-Paraffin-NBP1-06019-img0001.jpg "Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-06019] - Staining of Mouse Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Western Blot: SUR1 Antibody [NBP1-59778] - 293T cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/SUR1-Antibody-Western-Blot-NBP1-59778-img0005.jpg "Western Blot: SUR1 Antibody [NBP1-59778] - 293T cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP1-59778] - Human small intestine tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP1-59778-img0004.jpg)
![Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg)
![Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK](http://images.novusbio.com/fullsize/11-beta-HSD1-Antibody-Western-Blot-NBP2-27469-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK")
![<b>Genetic Strategies Validation. </b>Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/11-beta-HSD1-Antibody-Western-Blot-NBP2-27469-img0003.jpg)