| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52502-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37274-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97309SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47998-0.1mg | |||
| IHC-Prediluted | NBP2-48229 | |||
![Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COX-2-Antibody-2V7G8-Western-Blot-NBP3-16220-img0002.jpg "Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-155202485950..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249058..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Aanalysis of COX2/PTGS2 in paraffin-embedded mouse intestin tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249247..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of COX2/PTGS2 in paraffin-embedded rat colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249513.jpg)
![Western Blot: LDL R Antibody (1B10H10) [NBP2-52502] - Western blot analysis using LDLR mAb against human LDLR (AA: 22-150) recombinant protein. (Expected MW is 40.1 kDa)](http://images.novusbio.com/fullsize/LDL-R-Antibody-1B10H10-Western-Blot-NBP2-52502-img0003.jpg "Western Blot: LDL R Antibody (1B10H10) [NBP2-52502] - Western blot analysis using LDLR mAb against human LDLR (AA: 22-150) recombinant protein. (Expected MW is 40.1 kDa)")
![Immunohistochemistry: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis of paraffin-embedded ovarian cancertissues using LDLR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/LDL-R-Antibody-1B10H10-Immunohistochemistry-NBP2-52502-img0002.jpg)
![Flow Cytometry: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis of Hela cells using LDLR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/LDL-R-Antibody-1B10H10-Flow-Cytometry-NBP2-52502-img0001.jpg)
![Western Blot: LDL R Antibody (1B10H10) [NBP2-52502] - Analysis using LDLR mAb against HEK293 (1) and LDLR (AA: 22-150)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/LDL-R-Antibody-1B10H10-Western-Blot-NBP2-52502-img0004.jpg)
![ELISA: LDLR Antibody (1B10H10) [NBP2-52502] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/LDLR-Antibody-1B10H10-ELISA-NBP2-52502-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of bladder cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0005.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-ELISA-NBP2-37274-img0001.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Western-Blot-NBP2-73228-img0001.jpg "Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0005.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0002.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0004.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0006.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-278202415295584.jpg "Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29820241430414.jpg)
![Immunocytochemistry/ Immunofluorescence: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunocytochemistry analysis of HCT116 cells labeling dUTPase with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202410334031.jpg)
![Flow Cytometry: dUTPase Antibody (JE66-11) [NBP3-32281] - Flow cytometric analysis of HCT116 cells labeling dUTPase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32281, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202416372165.jpg)
![Immunohistochemistry-Paraffin: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NLS1317] - Analysis of anti-CYSLTR1 / CYSLT1 antibody with human lymph node, Hodgkins lymphoma.](http://images.novusbio.com/fullsize/Cysteinyl-Leukotriene-R1-CysLTR1-Antibody-Immunohistochemistry-Paraffin-NLS1317-img0002.jpg "Immunohistochemistry-Paraffin: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NLS1317] - Analysis of anti-CYSLTR1 / CYSLT1 antibody with human lymph node, Hodgkins lymphoma.")
![Immunohistochemistry-Paraffin: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NLS1317] - Analysis of anti-CYSLTR1 / CYSLT1 antibody with human nasal mucosa, allergic rhinitis, eosinophils.](http://images.novusbio.com/fullsize/Cysteinyl-Leukotriene-R1-CysLTR1-Antibody-Immunohistochemistry-Paraffin-NLS1317-img0001.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunohistochemistry-Paraffin-NBP2-67865-img0004.jpg)
![Flow Cytometry: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Flow-Cytometry-NBP2-67865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0002.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/AICDA-Antibody-Western-Blot-NBP1-30955-img0004.jpg "Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AICDA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0003.jpg)
![Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.](http://images.novusbio.com/fullsize/PAD4-Antibody-Immunohistochemistry-NBP1-97309-img0001.jpg "Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.")
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Western Blot Analysis of human liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Western-Blot-NBP2-44965-img0004.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Western Blot Analysis of human liver tissue lysate using Arginase 1/ARG1/liver Arginase antibody (ARG1/1125).")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Immunohistochemistry-Paraffin-NBP2-44965-img0001.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Western-Blot-NBP2-44965-img0002.jpg)
![Protein Array: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-ARG1-1125-Protein-Array-NBP2-44965-img0003.jpg)