| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92236-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89526-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-00093SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-159SS | |||
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar protein distribution across tissues to independent antibody NBP1-89526 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT2 Antibody [NBP1-89525] - Analysis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar pattern to independent antibody NBP1-85967 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP1-89525-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0019.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0020.jpg)
![Western Blot: SR-BI Antibody (SR37-06) [NBP3-11865] - Western blot analysis of SR-BI on different lysates using NBP3-11865 at 1/1,000 dilution. Positive control: Lane 1: Human liver. Lane 2: Mouse liver](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Western-Blot-NBP3-11865-img0001.jpg "Western Blot: SR-BI Antibody (SR37-06) [NBP3-11865] - Western blot analysis of SR-BI on different lysates using NBP3-11865 at 1/1,000 dilution. Positive control: Lane 1: Human liver. Lane 2: Mouse liver")
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunocytochemistry staining SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Immunocytochemistry-Immunofluorescence-NBP3-11865-img0006.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using NBP3-11865. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Immunohistochemistry-Paraffin-NBP3-11865-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunocytochemistry staining SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Immunocytochemistry-Immunofluorescence-NBP3-11865-img0005.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded human liver tissue using NBP3-11865. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Immunohistochemistry-Paraffin-NBP3-11865-img0002.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using NBP3-11865. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SR-BI-Antibody-SR37-06-Immunohistochemistry-Paraffin-NBP3-11865-img0003.jpg)
![Western Blot: OSBPL2 Antibody [NBP1-92236] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/OSBPL2-Antibody-Western-Blot-NBP1-92236-img0006.jpg "Western Blot: OSBPL2 Antibody [NBP1-92236] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: OSBPL2 Antibody [NBP1-92236] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/OSBPL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92236-img0008.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92236_rabbit-polyclonal-osbpl2-antibody-3052025958205.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89526-img0006.jpg "Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89526 (A) shows similar protein distribution across tissues to independent antibody NBP1-89525 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0013.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0015.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0014.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0016.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining in human lung and cerebral cortex tissues.. Corresponding MSR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining in human lung and cerebral cortex tissues.. Corresponding MSR1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0018.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0016.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining of human liver shows strong cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0020.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0015.jpg)
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody [NBP1-88125] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunohistochemistry-Paraffin-NBP1-88125-img0019.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0023.jpg "Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0016.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0019.jpg)
![Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Western-Blot-NBP1-00093-img0009.jpg "Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00093] - The SR-AI/MSR antibody was tested in Raw cells at a 1:50 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00093-img0008.jpg)
![Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Western-Blot-NB400-159-img0005.jpg "Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NB400-159] - Antibody was tested in Raw cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Immunocytochemistry-Immunofluorescence-NB400-159-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183319.jpg "Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183320.jpg)
![Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183336.jpg)
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183337.jpg)
![Western Blot: SR-BI Antibody (1C1I8) [NBP3-15311] - Western blot analysis of extracts of various cell lines, using SR-BI Rabbit mAb (NBP3-15311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/SR-BI-Antibody-1C1I8-Western-Blot-NBP3-15311-img0002.jpg "Western Blot: SR-BI Antibody (1C1I8) [NBP3-15311] - Western blot analysis of extracts of various cell lines, using SR-BI Rabbit mAb (NBP3-15311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: SR-BI Antibody (1C1I8) [NBP3-15311] - Immunohistochemistry of paraffin-embedded rat ovary using SR-BI Rabbit mAb (NBP3-15311) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SR-BI-Antibody-1C1I8-Immunohistochemistry-Paraffin-NBP3-15311-img0001.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-52202517184528.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-52202517223954.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-5220251723571.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-5220251726013.jpg)
![Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp2-04113_rabbit-polyclonal-scap-antibody-western-blot-2392024124054..jpg "Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:SCAP Antibody [NBP2-04113] -Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-SCAP antibody usedfor IP at 6 µg per reaction. SCAP was alsoimmunoprecipitated by a previous lot of this antibodyFor blotting immunoprecipitated SCAP,was used at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/antibody/nbp2-04113_rabbit-polyclonal-scap-antibody-immunoprecipitation-2392024131242..jpg)