| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52983SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44307SS | |||
| Azide and BSA Free | NBP2-80669 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77106-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85223-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71765 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| BSA Free | NBP3-07012 | |||
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0001.jpg "Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0006.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-52983] - Total protein from human HeLa cells, mouse 3T3 cells and rat PC12 cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um pore size PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Thioredoxin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-52983-img0003.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-52983] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse intestine using rabbit polyclonal antibody against Thioredoxin (1:300 dilution). The signal was developed with HRP-secondary -DAB method and the sections were counter-stained using hematoxylin. The staining was found primarily in the epithelial cells of the intestinal lumen with weak to negligible signal in the basal or muscular layers. Isolated epithelial cells depicted nuclear positivity also.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-52983-img0002.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - An intracellular stain was performed on HeLa cells with Thioredoxin-1 Antibody NBP2-52983AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-Flow-Cytometry-NBP2-52983-img0007.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - Thioredoxin Antibody [NBP2-52983] - An intracellular stain was performed on U-87 MG cells with Thioredoxin Antibody NBP2-52983 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Flow-Cytometry-NBP2-52983-img0004.jpg)
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunocytochemistry-Immunofluorescence-NBP2-44307-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern in the cytoplasm of cancer cells. Some cells depicted nuclear positivity also while the stroma was largely negative for CHREBP expression.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0003.jpg)
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern mainly in the cytoplasm (but in some nuclei also) of cuboidal epithelial cells of various ducts and tubules.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0002.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0005.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1005-img0001.jpg)
![Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NB100-1005-img0002.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0003.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0004.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Effects of FP on the expression of cholesterol metabolism-related genes and ABCA1 promoter activity in Caco-2 cells. After Caco-2 cells were treated with vehicle control or 1 mM FP for 24 h, total RNA and cell lysates were collected. ABCA1, NPC1L1, LXRalpha, and LXRbeta protein levels were analyzed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-56031-8) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue with LXR-A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunohistochemistry-Paraffin-NBP1-77106-img0002.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - LXR alpha/NR1H3 Antibody [NBP1-77106] - Rat liver tissue lysate at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NBP1-77106-img0004.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Rat liver tissue with LXR-A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunofluorescence-NBP1-77106-img0006.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419541563.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419313242.jpg)
![Immunohistochemistry: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunohistochemistry of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419553048.jpg)
![Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody - BSA Free [NBP1-77106] - Immunofluorescence of LXR alpha/NR1H3 in rat liver tissue with LXR alpha/NR1H3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77106_rabbit-polyclonal-lxr-alpha-nr1h3-antibody-2711202419432221.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-85223] - Analysis in human cell lines A-549 and HEK293 using Anti-NQO1 antibody. Corresponding NQO1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-85223-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-85223] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85223-img0008.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0017.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0015.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0016.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0018.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0009.jpg "Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunocytochemistry-Immunofluorescence-NBP2-01787-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: GADD34 Antibody (OTI3D12) [NBP2-01787] - SAHA/JQ1 treatment promotes UPR target oscillation. To demonstrate that YAP1 suppresses UPR in vivo, tumor sections were stained from allografts expressing control (scr) and Rela shRNA as well as KP, KPY, and SAHA/JQ1-treated KP tumors for the UPR target Gadd34, which was found to be upregulated in Yap1/NF-kappaB-deficient tumors. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1142-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-NBP2-01787-img0012.jpg)
![Flow Cytometry: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Flow-Cytometry-NBP2-01787-img0010.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0011.jpg)
![Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Western-Blot-NBP2-01787-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0005.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0006.jpg)
![Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Immunohistochemistry-Paraffin-NBP2-01787-img0007.jpg)
![Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Western-Blot-NBP2-33250-img0004.jpg "Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33250-img0002.jpg)
![Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33250-img0003.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0007.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0006.jpg)