| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38190-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38285-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59483-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85218-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92202-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55813-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30612-25ul | |||
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241252813.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human colon using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241233823.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP3-38190] - Western blot analysis of various lysates using Aspartate Aminotransferase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-1012202411592816.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-10122024120650.jpg)
![Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunofluorescence analysis of U2OS cells using Aspartate Aminotransferase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241243928.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining in human parathyroid gland and pancreas tissues using anti-IMMT antibody. Corresponding IMMT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Mitofilin Antibody [NBP2-38285] - Analysis using Anti-IMMT antibody NBP2-38285 (A) shows similar pattern to independent antibody NBP2-38286 (B).](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NBP2-38285-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NBP2-38285] - Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38285-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0005.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NBP2-38285] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NBP2-38285-img0006.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0009.jpg "Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2")
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Immunocytochemistry-Immunofluorescence-NBP2-59483-img0002.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0006.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Lane 1: 293T cell lysates, Lane 2: GOT1 Transfected 293T cell lysates](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0001.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0008.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0003.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0007.jpg "Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0002.jpg)
![Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0003.jpg)
![Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining in human kidney and skeletal muscle tissues using anti-BDH2 antibody. Corresponding BDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0005.jpg)
![Immunohistochemistry-Paraffin: BDH2 Antibody [NBP1-85218] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-85218-img0006.jpg)
![Western Blot: BDH2 Antibody [NB100-93379] - HEK293 overexpressing BDH2 and probed (mock transfection in first lane).](http://images.novusbio.com/fullsize/BDH2-Antibody-Western-Blot-NB100-93379-img0002.jpg "Western Blot: BDH2 Antibody [NB100-93379] - HEK293 overexpressing BDH2 and probed (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: BDH2 Antibody [NB100-93379] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BDH2-Antibody-Immunohistochemistry-Paraffin-NB100-93379-img0003.jpg)
![Western Blot: BDH2 Antibody [NB100-93379] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/BDH2-Antibody-Western-Blot-NB100-93379-img0001.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NB100-93403-img0003.jpg "Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0002.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0001.jpg)
![Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Western-Blot-NBP2-92202-img0002.jpg "Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ACSS1 Antibody [NBP2-92202] - Analysis of L929 cells using ACSS1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92202-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-92202] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (NBP2-92202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-92202-img0003.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Aspartate Aminotransferase Antibody [NBP2-55813] - Analysis using Anti-GOT1 antibody NBP2-55813 (A) shows similar pattern to independent antibody NBP2-57518 (B).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP2-55813-img0006.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0003.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0004.jpg)
![Western Blot: ACSS1 Antibody [NBP2-30612] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/ACSS1-Antibody-Western-Blot-NBP2-30612-img0002.jpg "Western Blot: ACSS1 Antibody [NBP2-30612] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-30612] - Staining in human placenta and liver tissues using anti-ACSS1 antibody. Corresponding ACSS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-30612-img0006.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-30612] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-30612-img0004.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-30612] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-30612-img0005.jpg)