| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59724SS | |||
| Azide and BSA Free | NBP2-80643 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52983SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71189 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52508-0.025mg | |||
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0011.jpg "Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.")
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0012.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified customer review.](http://images.novusbio.com/fullsize/iNOS-Antibody-K13-A-Immunohistochemistry-Paraffin-NBP1-33780-img0010.jpg)
![Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, & Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35391753), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33780_rabbit-monoclonal-inos-antibody-k13-a-31020241535639.jpg)
![Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Paraffin-NB600-1423-img0003.jpg "Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human")
![Flow Cytometry: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-Antibody-FA6-152-Flow-Cytometry-NB600-1423-img0007.jpg)
![Immunohistochemistry-Frozen: CD36 Antibody (FA6-152) [NB600-1423] - Frozen section of tonsil tissue. Staining especially in vascular endothelia.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Frozen-NB600-1423-img0001.jpg)
![Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Western-Blot-NB110-59724-img0007.jpg "Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.")
![Immunohistochemistry: CD36 Antibody (D-2712) [NB110-59724] - Staining of CD36 in mouse liver.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Immunohistochemistry-NB110-59724-img0006.jpg)
![Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-2-Antibody-Western-Blot-NB100-868-img0007.jpg "Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasm and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-868-img0006.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868] - 10x image of mouse lung tissue expressing cox-2. Secondary antibody: Alexa Fluor 546 (red), counterstain: DAPI (blue). Goat anti-mouse cox-2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections. Antigen retrieval in citrate buffer (95C, 20 min) followed by permeabilization with 0.2% Triton X-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-868-img0008.jpg)
![Flow Cytometry: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line)](http://images.novusbio.com/fullsize/COX-2-Antibody-Flow-Cytometry-NB100-868-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml, showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue)](http://images.novusbio.com/fullsize/COX-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-868-img0005.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6 Western Blot analysis of SPHK1 expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0002.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6.Lane 1: SPHK1 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0006.jpg)
![ELISA: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-ELISA-H00008877-M01-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0001.jpg "Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0006.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-52983] - Total protein from human HeLa cells, mouse 3T3 cells and rat PC12 cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um pore size PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Thioredoxin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-52983-img0003.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-52983] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse intestine using rabbit polyclonal antibody against Thioredoxin (1:300 dilution). The signal was developed with HRP-secondary -DAB method and the sections were counter-stained using hematoxylin. The staining was found primarily in the epithelial cells of the intestinal lumen with weak to negligible signal in the basal or muscular layers. Isolated epithelial cells depicted nuclear positivity also.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-52983-img0002.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - An intracellular stain was performed on HeLa cells with Thioredoxin-1 Antibody NBP2-52983AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-Flow-Cytometry-NBP2-52983-img0007.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - Thioredoxin Antibody [NBP2-52983] - An intracellular stain was performed on U-87 MG cells with Thioredoxin Antibody NBP2-52983 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Flow-Cytometry-NBP2-52983-img0004.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD36-Antibody-1283D-BSA-Free-Western-Blot-NBP2-54790-img0024.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0022.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.")
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded prostate tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0020.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Leukotriene A4 hydrolase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Leukotriene A4 hydrolase.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0021.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0013.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0014.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded endometrium tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0018.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0017.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded pancreas tissue using anti-Leukotriene A4 hydrolasemouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0019.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunoprecipitation-NBP1-47829-img0023.jpg)
![Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Western-Blot-NBP2-52508-img0007.jpg "Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)")
![Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunohistochemistry-NBP2-52508-img0006.jpg)
![Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Flow-Cytometry-NBP2-52508-img0001.jpg)
![Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against HEK293 (1) and MAP2K3 (AA: 1-138)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Western-Blot-NBP2-52508-img0008.jpg)
![Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunohistochemistry-Paraffin-NBP2-52508-img0005.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of GC-7901 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0002.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0003.jpg)
![Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of HepG2 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-Immunofluorescence-NBP2-52508-img0004.jpg)
![ELISA: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-2E12D11-ELISA-NBP2-52508-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0005.jpg "Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.")
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0006.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis (surface staining) of human peripheral blood with anti-human CD38 (HIT2) Pacific Blue(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0012.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Analysis using the PE conjugate of NB500-510. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0002.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0003.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) FITC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0004.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0007.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) PerCP.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0008.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0009.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) APC.](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0010.jpg)
![Flow Cytometry: CD38 Antibody (HIT2) [NB500-510] - Human peripheral blood with anti-human CD38 (HIT2) Pacific Orange(TM).](http://images.novusbio.com/fullsize/CD38-Antibody-HIT2-Flow-Cytometry-NB500-510-img0011.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0020.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta, prostate, skeletal muscle and testis using Anti-B4GALT1 antibody NBP1-88654 (A) shows similar protein distribution across tissues to independent antibody NBP1-88655 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Analysis in human cell lines HeLa and HEK293 using anti-B4GALT1 antibody. Corresponding B4GALT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Western-Blot-NBP1-88654-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88654-img0007.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0023.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody [NBP1-88654] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/beta-1-4-Galactosyltransferase-1-B4GalT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88654-img0022.jpg)