| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-164SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44307SS | |||
| Azide and BSA Free | NBP2-80669 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54792SS | |||
| Azide and BSA Free | NBP2-80556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73152 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70068 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-136SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-107SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05950SS | |||
![Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0006.jpg "Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.")
![Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-106-img0002.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression and activity in RA and OA joints and 3OH-myristate levels in synovial fluid of RA and OA patients. Immunohistological analysis of PLTP expression in synovial tissue from 5 RA and 6 OA patients stained for PLTP and macrophages (CD68+ cells); All RA synovial tissue showed positive stainings while only 2 out 6 OA tissues were positive for PLTP staining. Representative stainings are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0008.jpg)
![Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0001.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0005.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-Paraffin-NB400-106-img0007.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-106_rabbit-polyclonal-pltp-antibody-310202416171467.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of FFPE mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Immunohistochemistry-Paraffin-NB400-164-img0007.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB400-164 at various dilutions in ABCA1 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of ABCA1 in (A) T09 treated RAW 264.7 cells and (B) untreated RAW 264.7 cells.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0006.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation downregulates hepatic Abca1 levels.(A) Western blot analysis of Abca1 expression in the liver from Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with HCHF diet for 16 weeks (n = 5, each group). (B) Western blot analysis of Abca1 expression in the liver from 16 week HCHF-fed Lrp1Y63F;Ldlr−/− & Ldlr−/− mice after BMT (n = 5, each group). All data are mean ±SEM. **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241535616.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416175236.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F impairs Abca1 induction through inhibiting the PPAR gamma /LXR pathway.(A) Real-time PCR analysis of Abca1, Abca7, Pparg, Nr1h3 & Nr2h2 expression in macrophages treated with or without ox-LDL. (B–E) Isolated peritoneal macrophages from Lrp1Y63F;Ldlr−/− & Ldlr−/− were pre-incubated for 1 hr with (B) T0070907 (PPAR gamma antagonist, 10 μM), (C) Rosiglitizone (PPAR gamma agonist) at indicated concentrations in μM, (D) T0901317 (nonspecific LXR agonist) at indicated concentrations in μM, (E) LXR623 (LXR beta full agonist & LXR alpha partial agonist) at indicated concentrations in μM, followed by the treatment with or without oxLDL for an additional 24 hr. Abca1 expression was assessed by immunoblot analysis (left). Protein expression was quantified & analyzed (right). Data are mean ±SEM from three independent experiments. *p<0.05; **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416154639.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241616063.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunocytochemistry-Immunofluorescence-NBP2-44307-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHREBP Antibody (2D9NB) [NBP2-44307] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CHREBP (2D9NB) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of human hepatocellular carcinoma using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern in the cytoplasm of cancer cells. Some cells depicted nuclear positivity also while the stroma was largely negative for CHREBP expression.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0003.jpg)
![Immunohistochemistry-Paraffin: CHREBP Antibody (2D9NB) [NBP2-44307] - Analysis of a FFPE tissue section of normal human kidney using 1:200 dilution of CHREBP antibody (clone 2D9NB). The antibody generated a diffused to punctate staining pattern mainly in the cytoplasm (but in some nuclei also) of cuboidal epithelial cells of various ducts and tubules.](http://images.novusbio.com/fullsize/CHREBP-Antibody-2D9NB-Immunohistochemistry-Paraffin-NBP2-44307-img0002.jpg)
![Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Western-Blot-NBP2-54792-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0003.jpg)
![Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Flow-Intracellular-NBP2-54792-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0002.jpg)
![Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0005.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Western-Blot-NBP2-00935-img0002.jpg "Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.")
![Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (1F2) [NBP2-00935] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunocytochemistry-Immunofluorescence-NBP2-00935-img0014.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (OTI1F2) [NBP2-00935] - Staining of paraffinembedded Adenocarcinoma of Humanendometrium tissue using anti-OSBP mousemonoclonal antibody. (Heat-induced epitoperetrieval by 10mM citric buffer, pH6.0, 100 degrees C for10min, TA503140)](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0015.jpg)
![Flow Cytometry: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OSBP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Flow-Cytometry-NBP2-00935-img0001.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0003.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0004.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0005.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0006.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0007.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0008.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0009.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human endometrium tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0010.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human Kidney tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0011.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0012.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0013.jpg)
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0008.jpg "Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunocytochemistry-Immunofluorescence-NBP2-02588-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0006.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0002.jpg)
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0004.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0005.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - Analysis of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHREBP Antibody [NB400-136] - Staining of a human hepatocyte using NB400-136. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).](http://images.novusbio.com/fullsize/CHREBP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-136-img0002.jpg "Immunocytochemistry/Immunofluorescence: CHREBP Antibody [NB400-136] - Staining of a human hepatocyte using NB400-136. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).")
![Western Blot: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Western-Blot-NBP2-73152-img0001.jpg "Western Blot: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.")
![Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73152-img0003.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0014.jpg)
![Flow Cytometry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-OSBP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73152-img0002.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0004.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0005.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0006.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0007.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0008.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0009.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0010.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Human endometrium tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0011.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Human Kidney tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0012.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin-embedded Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0013.jpg)
![Immunohistochemistry: OSBP1 Antibody (OTI1F2) - Azide and BSA Free [NBP2-73152] - Staining of paraffin embedded Adenocarcinoma of Human](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73152-img0015.jpg)
![Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.](http://images.novusbio.com/fullsize/LCAT-Antibody-Western-Blot-NBP1-05950-img0003.jpg "Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.")
![Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/LCAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05950-img0004.jpg)