| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24950SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9216-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82048-0.025ml | |||
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0002.jpg "Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.")
![Immunocytochemistry/Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70739-img0005.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0004.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0001.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0003.jpg)
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")
![ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-32-BSA-Free-ELISA-NBP1-05182-img0001.jpg "ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.")
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)
![Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0006.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16307-img0003.jpg)
![Immunoprecipitation: 4EBP1 Antibody [NBP2-16307] - Immunoprecipitation of 4EBP1 protein from HCT116 whole cell extracts using 5 ug of 4EBP1 antibody or 4E-BP1 antibody. Western blot analysis was performed using 4EBP1 antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunoprecipitation-NBP2-16307-img0005.jpg)
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)
![Western Blot: Nogo B receptor Antibody [NBP2-24950] - Western blot analysis of Nogo-Breceptor in A) human, B) mouse and C) rat brain tissue lysate using NBP2-24950 at 2ug/ml.](http://images.novusbio.com/fullsize/Nogo-B-receptor-Antibody-Western-Blot-NBP2-24950-img0002.jpg "Western Blot: Nogo B receptor Antibody [NBP2-24950] - Western blot analysis of Nogo-Breceptor in A) human, B) mouse and C) rat brain tissue lysate using NBP2-24950 at 2ug/ml.")
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Analysis of HepG2, Daudi and MOLT-4 cells lysates with 0.5 ug/ml of hLDHA/LDHB Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hLDHA/LDHB at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2066A-Western-Blot-MAB9216-img0001.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Analysis of HepG2, Daudi and MOLT-4 cells lysates with 0.5 ug/ml of hLDHA/LDHB Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hLDHA/LDHB at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Lactate Dehydrogenase A/LDHA Antibody (2066A) [MAB9216] - Lactate Dehydrogenase A/LDHA was detected in immersion fixed PC3 cell using Lactate Dehydrogenase A/LDHA Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using Donkey anti-Rabbit IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody-2066A-Immunocytochemistry-MAB9216-img0002.jpg)
![Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Western-Blot-NB100-82048-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Staining of methanol-fixed Hela cells using NB100-82048](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82048-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) antibody](http://images.novusbio.com/fullsize/MKK3-MEK3-[p-Ser189]-Antibody-Western-Blot-NB100-82048-img0007.jpg)
![Immunohistochemistry-Paraffin: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82048(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-82048_rabbit-polyclonal-mkk3-mek3-p-ser189-antibody-103202523464532.jpg)
![Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - Analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.Lane 1: FHL1 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FHL1-Antibody-2A9-Western-Blot-H00002273-M01-img0010.jpg "Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - Analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.Lane 1: FHL1 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in PC-12.](http://images.novusbio.com/fullsize/FHL1-Antibody-2A9-Western-Blot-H00002273-M01-img0007.jpg)
![Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/FHL1-Antibody-2A9-Western-Blot-H00002273-M01-img0008.jpg)
![Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/FHL1-Antibody-2A9-Western-Blot-H00002273-M01-img0009.jpg)
![Sandwich ELISA: FHL1 Antibody (2A9) [H00002273-M01] - Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FHL1-Antibody-2A9-Sandwich-ELISA-H00002273-M01-img0006.jpg)
![Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Western-Blot-H00005606-M02-img0010.jpg "Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of monoclonal antibody to MAP2K3 on formalin-fixed paraffin-embedded human dysgerminoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Immunohistochemistry-Paraffin-H00005606-M02-img0007.jpg)
![Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Western Blot analysis of MAP2K3 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Western-Blot-H00005606-M02-img0001.jpg)
![Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 transfected lysate using anti-MAP2K3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAP2K3 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Immunoprecipitation-H00005606-M02-img0008.jpg)
![Sandwich ELISA: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKK3-MEK3-Antibody-1D10-Sandwich-ELISA-H00005606-M02-img0009.jpg)
![SDS-Page: Recombinant Human Caveolin-1 Protein [H00000857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Caveolin-1-Protein-SDS-Page-H00000857-P01-img0002.jpg "SDS-Page: Recombinant Human Caveolin-1 Protein [H00000857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")