| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97369-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00064-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99633-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00295-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98977-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90222-50ul | |||
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-278202415141717.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Immunocytochemistry analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1 with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202410234298.jpg)
![Flow Cytometry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Flow cytometric analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32019, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202416453266.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-298202414333655.jpg "Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 65 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31996) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-392024848509.jpg)
![Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-278202416331270.jpg "Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (8D3-R) [NBP3-32054] - Immunocytochemistry analysis of HeLa cells labeling ATF6 with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-29202410234226.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97369-img0002.jpg "Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Derlin 1 Antibody - BSA Free [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with NBP2-97369 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-235202514492372.png)
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP2-97369] - Immunofluorescence staining of Derlin 1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97369 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-23520252135188.png)
![Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0006.jpg)
![Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - ADP-Sugar Pyrophosphatase/NUDT5 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Immunoprecipitation-NBP3-00064-img0004.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-99633-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-99633] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate. 4 ul anti-14-3-3 epsilon rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 epsilon rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-99633-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-99633] - Immunofluorescence staining of 14-3-3 epsilon in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99633 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99633_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511444474.png)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human lung cancer with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-235202514331511.png "Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human lung cancer with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP3-00295] - Immunochemical staining of human FHIT in human breast carcinoma with NBP3-00295 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-23520251633505.png)
![Immunocytochemistry/ Immunofluorescence: FHIT Antibody [NBP3-00295] - Immunofluorescence staining of FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00295 (1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp3-00295_rabbit-polyclonal-fhit-antibody-23520251129352.png)
![Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-278202416331235.jpg "Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAP3 Antibody (PSH01-34) [NBP3-32526] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-298202414333666.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-278202416331212.jpg "Western Blot: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32721) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (JE40-24) [NBP3-32721] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32721_rabbit-pyruvate-carboxylase-mab-je40-24-2982024142096.jpg)
![Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-27820241628527.jpg "Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH04-52) [NBP3-32546] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-298202414262677.jpg)
![Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP2-98977-img0003.jpg "Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa")
![Immunoprecipitation: ABCF2 Antibody [NBP2-98977] - ABCF2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 1 ul anti-ABCF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ABCF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunoprecipitation-NBP2-98977-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NBP2-98977] - Immunofluorescence staining of ABCF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98977 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98977_rabbit-polyclonal-abcf2-antibody-23520251125448.png)
![Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Western-Blot-NBP2-90222-img0004.jpg "Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa")
![Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 25 kDa. Observed band size: 25 kDa.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-001-Immunoprecipitation-NBP2-90222-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90222 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-90222_rabbit-monoclonal-phosphoserine-phosphatase-antibody-001-235202511444476.png)