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![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Western-Blot-NBP2-80036-img0006.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunocytochemistry-Immunofluorescence-NBP2-80036-img0004.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0002.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15 ug/mL).](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunohistochemistry-Paraffin-NBP2-80036-img0001.jpg)
![PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1:2000 dilution. 1. Human Nampt (Visfatin/PBEF) (His-tagged). 2. Human Nampt (Visfatin/PBEF) (FLAG(R)-tagged). 3. Mouse Nampt (Visfatin/PBEF) (His-tagged). 4. Rat Nampt (Visfatin/PBEF) (His-tagged). 5. Human Nampt (Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-NBP2-80036-img0005.jpg)
![Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt (Visfatin/PBEF) pAb.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-OMNI379-Immunoprecipitation-NBP2-80036-img0007.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Western-Blot-NBP2-76368-img0004.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE NCI-H460 by ICC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunocytochemistry-NBP2-76368-img0003.jpg)
![Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunohistochemistry-NBP2-76368-img0002.jpg)
![Flow Cytometry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Rabbit antiNAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Flow-Cytometry-NBP2-76368-img0006.jpg)
![Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunohistochemistry-NBP2-76368-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368), rabbit anti-NAMPT/PBEF/Visfatin antibodies NB100-594, and one other rabbit anti-NAMPT/PBEF/Visfatin antibody.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-BLR058F-Immunoprecipitation-NBP2-76368-img0005.jpg)
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19468] - Paraffin-embedded rat liver. Visfatin antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NBP2-19468-img0006.jpg "Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NBP2-19468] - Paraffin-embedded rat liver. Visfatin antibody [N1N3] diluted at 1:500.")
![Western Blot: NAMPT Antibody [NBP2-19468] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NAMPT-Antibody-Western-Blot-NBP2-19468-img0001.jpg)
![ELISA: Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76712] - Samples were spiked with high concentrations of Rat PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76712_rat-pbef-visfatin-nampt-elisa-kit-colorimetric-132202416133635.jpg "ELISA: Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76712] - Samples were spiked with high concentrations of Rat PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76712] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76712_rat-pbef-visfatin-nampt-elisa-kit-colorimetric-432024955031.jpg)
![ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76710] - Samples were spiked with high concentrations of Human PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76710_human-pbef-visfatin-nampt-elisa-kit-colorimetric-132202416175675.jpg "ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76710] - Samples were spiked with high concentrations of Human PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76710] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76710_human-pbef-visfatin-nampt-elisa-kit-colorimetric-432024915899.jpg)
![ELISA: Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76711] - Samples were spiked with high concentrations of Mouse PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76711_mouse-pbef-visfatin-nampt-elisa-kit-colorimetric-13220241692260.jpg "ELISA: Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76711] - Samples were spiked with high concentrations of Mouse PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76711] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76711_mouse-pbef-visfatin-nampt-elisa-kit-colorimetric-43202490355.jpg)
![SDS-Page: Recombinant Mouse PBEF/Visfatin/NAMPT Protein [NBC1-22597] - Visfatin, 57 kDa (511 aa) , confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/NAMPT-Protein-SDS-Page-NBC1-22597-img0001.jpg "SDS-Page: Recombinant Mouse PBEF/Visfatin/NAMPT Protein [NBC1-22597] - Visfatin, 57 kDa (511 aa) , confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")
![ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683] - Sample standard curve](http://images.novusbio.com/fullsize/Human-PBEF-Visfatin-NAMPT-ELISA-Kit-Colorimetric-ELISA-NBP3-11683-img0001.jpg "ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683] - Sample standard curve")