| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78782SS | |||
| Azide and BSA Free | NBP2-80714 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72117 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-63255-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65729SS | |||
![Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249351317.jpg "Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249425493.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-212024941472.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249383189.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249355579.jpg)
![Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-78132-img0002.jpg "Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-Paraffin-NBP2-78132-img0001.jpg)
![Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Rat plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0005.jpg "Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Rat plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody [NBP3-13331] - Rat thymus gland. Complement Component C1qA stained by Complement Component C1qA antibody (NBP3-13331) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-Immunohistochemistry-Paraffin-NBP3-13331-img0002.jpg)
![Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Mouse plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0001.jpg)
![Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Human plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0004.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Analysis in human testis and pancreas tissues using antibody. Corresponding CLEC16A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-3820238318..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Analysis in human testis and pancreas tissues using antibody. Corresponding CLEC16A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202383247..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202383534..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202384025..jpg)
![Immunohistochemistry-Paraffin: CLEC16A Antibody [NBP1-81805] -Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-81805_rabbit-polyclonal-clec16a-antibody-immunohistochemistry-paraffin-38202384141..jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (HL1033) [NBP3-13687] - MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/MBP-Antibody-HL1033-Immunohistochemistry-Paraffin-NBP3-13687-img0002.jpg "Immunohistochemistry-Paraffin: MBP Antibody (HL1033) [NBP3-13687] - MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:100.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-910202420122087.jpg)
![Myelin basic protein antibody [HL1033] detects Myelin basic protein protein by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.Myelin basic protein stained by Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-immunohistochemistry-paraffin-261120251834262.jpg)
![Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-western-blot-261120251829269.jpg)
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [HL1033] (NBP3-13687) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-western-blot-261120251831289.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Western-Blot-NBP2-78782-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) at 3 ug/mL for 1 hour at room temperature followed by incubation with an anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Immunohistochemistry-Paraffin-NBP2-78782-img0002.jpg)
![Immunohistochemistry-Paraffin: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - C1 inhibitor antibody [HL1196] detects secreted C1 inhibitor protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. C1 inhibitor stained by C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25387_rabbit-serpin-g1-c1-inhibitor-mab-hl1196-azide-bsa-free-2120249432810.jpg "Immunohistochemistry-Paraffin: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - C1 inhibitor antibody [HL1196] detects secreted C1 inhibitor protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. C1 inhibitor stained by C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - C1 inhibitor antibody [HL1196] detects C1 inhibitor protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. C1 inhibitor stained by C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25387_rabbit-serpin-g1-c1-inhibitor-mab-hl1196-azide-bsa-free-2120249403885.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - Mouse plasma and rat plasma (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25387_rabbit-serpin-g1-c1-inhibitor-mab-hl1196-azide-bsa-free-212024939188.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - C1 inhibitor antibody [HL1196] detects C1 inhibitor protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 10 min. Green: C1 inhibitor stained by C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25387_rabbit-serpin-g1-c1-inhibitor-mab-hl1196-azide-bsa-free-2120249393589.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (HL1196) - Azide and BSA Free [NBP3-25387] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C1 inhibitor antibody [HL1196] (NBP3-25387) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25387_rabbit-serpin-g1-c1-inhibitor-mab-hl1196-azide-bsa-free-2120249374915.jpg)
![Western Blot: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE-2.](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Western-Blot-NBP2-46490-img0005.jpg "Western Blot: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE-2.")
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0004.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0001.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0002.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0011.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.")
![Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunohistochemistry-Frozen-NB200-610-img0018.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0007.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0001.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0008.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Flow-Cytometry-NB100-63255-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD29.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Flow-Cytometry-NB100-63255-img0002.jpg)
![Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD8-Antibody-4B11-Dual-RNAscope-ISH-IHC-NB100-65729-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody (4B11) [NB100-65729] - Analysis of formalin fixed, paraffin-embedded human tonsil with CD8 Antibody (4B11). Magnification: 40X.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-4B11-Immunohistochemistry-Paraffin-NB100-65729-img0002.jpg)