| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-27946SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36495SS | |||
| Azide and BSA Free | NBP2-80632 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-75925SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-50056-20ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44541 | |||
| Azide and BSA Free | NBP2-34587-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43871 | |||
| BSA Free | NBP3-07981-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18333-0.025mg | |||
![Western Blot: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of (A) partial recombinant 15 kDa human CD163 protein and (B) human lungs lysate with 2 ug/ml concentration of CD163 antibody clone 6D3.2F3](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Western-Blot-NBP2-36495-img0001.jpg "Western Blot: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of (A) partial recombinant 15 kDa human CD163 protein and (B) human lungs lysate with 2 ug/ml concentration of CD163 antibody clone 6D3.2F3")
![Immunohistochemistry-Paraffin: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of CD163 antibody (clone 6D3.2F3). CD163 is a histiocytic marker and in the tested placental tissue section, this antibody generated a specific staining in the Hofbauer cells which are eosinophilic histiocytes found in placenta.](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Immunohistochemistry-Paraffin-NBP2-36495-img0003.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (6D3.2F3) [NBP2-36495] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of CD163 antibody (clone 6D3.2F3). CD163 is a histiocytic marker and in the tested placental tissue section, this antibody generated a specific staining in the Hofbauer cells which are eosinophilic histiocytes found in placenta.](http://images.novusbio.com/fullsize/CD163-Antibody-6D3-2F3-Immunohistochemistry-Paraffin-NBP2-36495-img0002.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0005.jpg "Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry: MIF Antibody [NB100-1789] - Co-localization of CXCR4 and MIF in urothelium. Sections from rats treated with saline or CYP are shown. MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) and an overlay panel combining both immunostaining and a DAPI nuclear stain. MIF immunostaining is seen in basal and intermediate cells and in fibroblasts in the lamina propria of saline treated rats, while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal and intermediate cells of urothelium. CYP treatment resulted in superficial cell staining for MIF and CXCR4 and overlay panels demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar=50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003898) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-NB100-1789-img0006.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Daudi (A) and Jurkat (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0003.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NB100-1789] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NB100-1789-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MIF Antibody [NB100-1789] - Co-localization of CXCR4 & MIF in urothelium.Representative sections from rats treated with saline (A–C) or CYP (D–I) are shown. The figure shows MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) & an overlay panel combining both immunostaining & a DAPI nuclear stain. MIF immunostaining is seen in basal & intermediate cells & in fibroblasts in the lamina propria of saline treated rats (A), while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal & intermediate cells of urothelium (B) & lamina propria is not stained. Overlay of these panels (C) demonstrate co-localization of MIF & CXCR4 as orange coloring of cells. CYP treatment resulted in superficial cell staining for MIF (D,G) & CXCR4 (E,H) & overlay panels (F,I) demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19066630), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1789_goat-polyclonal-mif-antibody-31020241554531.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0014.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0002.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0003.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0006.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0008.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0009.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0010.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0011.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of human peripheral blood with anti-CD16 (3G8) FITC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0012.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0013.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Tonsil stained with .](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0006.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Tonsil stained with .")
![Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Western-Blot-NBP2-32832-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Immunofluorescence staining of U87MG cells using CD68/SR-D1 Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunocytochemistry-Immunofluorescence-NBP2-32832-img0004.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Flow Cytometric Analysis of U87MG cells using CD68/SR-D1 Antibody (C68/684).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Flow-Cytometry-NBP2-32832-img0008.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68/SR-D1 Antibody (C68/684).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0005.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Immunohistochemistry-Frozen: CD163 Antibody (RM3/1) [NBP1-60158] - Staining of interfollicular tissue in frozen tonsil sections.](http://images.novusbio.com/fullsize/CD163-Antibody-RM3-1-Immunohistochemistry-Frozen-NBP1-60158-img0001.jpg "Immunohistochemistry-Frozen: CD163 Antibody (RM3/1) [NBP1-60158] - Staining of interfollicular tissue in frozen tonsil sections.")
![Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-212024940820.jpg "Immunohistochemistry-Paraffin: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249425467.jpg)
![Western Blot: TREM2 Antibody (HL1738) - Azide and BSA Free [NBP3-25728] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-2120249422617.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (1 ug/mL)Please notice that NBP3-25728 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518443414.jpg)
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (NBP3-25728) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-1411202518414124.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (NBP3-25728) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-western-blot-14112025184488.jpg)
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (NBP3-25728) (5 ug/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (1 ug/mL)Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25728. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25728_rabbit-trem2-mab-hl1738-azide-and-bsa-free-elisa-141120251843335.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-07981 antibody. Corresponding CD163 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-07981 antibody. Corresponding CD163 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD163 Antibody (CL10652) [NBP3-07981] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Western-Blot-NBP3-07981-img0008.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in perivascular macrophages.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0007.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human cerebellum shows moderate cytoplasmic positivity in microglia and strong staining in perivascular macrophages.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0002.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human cerebral cortex (Alzheimer's disease) shows strong cytoplasmic positivity in perivascular macrophages and in microglia.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0003.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human liver shows strong cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0004.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0005.jpg)
![Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD163-Antibody-CL10652-Immunohistochemistry-Paraffin-NBP3-07981-img0006.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0001.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL")
![Western Blot: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation in Mouse Tissues.Loading: 15 ug of lysates per lane. Antibodies: ZC3H12A , 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419241348.gif)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0003.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419381765.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419541514.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419475869.jpg)
![Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling MCPIP1/ZC3H12A with NBP3-18333 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18333-img0004.jpg)