| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89068-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87184-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79840 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41326-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92832-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-91995-0.02ml | |||
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0007.jpg "Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human kidney shows strong membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0005.jpg)
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human endometrium shows no membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0006.jpg)
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human placenta shows moderate membranous and cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0008.jpg)
![Western Blot: ABP1/AOC1 Antibody [NBP1-89068] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/antibody/nbp1-89068_rabbit-polyclonal-abp1-aoc1-antibody-western-blot-98202491345..jpg)
![Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis using Anti-CRP antibody NBP1-87184 (A) shows similar pattern to independent antibody NBP1-87183 (B).](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Western-Blot-NBP1-87184-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis using Anti-CRP antibody NBP1-87184 (A) shows similar pattern to independent antibody NBP1-87183 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human tonsil shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0011.jpg)
![Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis in human plasma.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Western-Blot-NBP1-87184-img0005.jpg)
![Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human duodenum shows moderate positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0009.jpg)
![Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0010.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA DQ/DR/DP antibody (rHLA-Pan/3475).](http://images.novusbio.com/fullsize/HLA-DQ-DR-DP-Antibody-rHLA-Pan-3475-Western-Blot-NBP3-07808-img0004.jpg "Western Blot: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA DQ/DR/DP antibody (rHLA-Pan/3475).")
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Immunofluorescence Analysis of Human Raji cells with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).](http://images.novusbio.com/fullsize/HLA-DQ-DR-DP-Antibody-rHLA-Pan-3475-Immunocytochemistry-Immunofluorescence-NBP3-07808-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475).](http://images.novusbio.com/fullsize/HLA-DQ-DR-DP-Antibody-rHLA-Pan-3475-Immunohistochemistry-Paraffin-NBP3-07808-img0003.jpg)
![Flow Cytometry: HLA DQ/DR/DP Antibody (rHLA-Pan/3475) [NBP3-07808] - Flow Cytometric Analysis of Human Raji cells using HLA DQ/DR/DP Mouse Recombinant Monoclonal Antibody (rHLA DQ/DR/DP/3475)followed by goat anti- Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/HLA-DQ-DR-DP-Antibody-rHLA-Pan-3475-Flow-Cytometry-NBP3-07808-img0001.jpg)
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Western Blot: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Western-Blot-NBP2-79706-img0004.jpg "Western Blot: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).")
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Formalin-fixed, paraffin-embedded human Spleen stained with CD3 epsilon Antibody (C3e/1931).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Immunohistochemistry-Paraffin-NBP2-79706-img0002.jpg)
![Flow Cytometry: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon Antibody (C3e/1931) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Flow-Cytometry-NBP2-79706-img0001.jpg)
![Protein Array: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD3 epsilon Antibody (C3e/1931) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Protein-Array-NBP2-79706-img0003.jpg)
![Western Blot: ORAI3 Antibody (2H2G9) [NBP2-41326] - Analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ORAI3-Antibody-2H2G9-Western-Blot-NBP2-41326-img0003.jpg "Western Blot: ORAI3 Antibody (2H2G9) [NBP2-41326] - Analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody (2H2G9) [NBP2-41326] - Staining of ORAI3 in human spleen tissue with ORAI3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ORAI3-Antibody-2H2G9-Immunocytochemistry-Immunofluorescence-NBP2-41326-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody (2H2G9) - BSA Free [NBP2-41326] - Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41326_mouse-monoclonal-orai3-antibody-2h2g9-2711202419313221.jpg)
![Immunohistochemistry: ORAI3 Antibody (2H2G9) - BSA Free [NBP2-41326] - Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41326_mouse-monoclonal-orai3-antibody-2h2g9-2711202420392724.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-2711202419381793.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241933343.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM , (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241928862.gif)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.")
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-40799-img0005.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0006.jpg)
![Western Blot: AP1M2 Antibody [NBP2-92832] - Analysis of extracts of various cell lines, using AP1M2 .Exposure time: 1s.](http://images.novusbio.com/fullsize/AP1M2-Antibody-Western-Blot-NBP2-92832-img0003.jpg "Western Blot: AP1M2 Antibody [NBP2-92832] - Analysis of extracts of various cell lines, using AP1M2 .Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: AP1M2 Antibody - BSA Free [NBP2-92832] - Immunofluorescence analysis of U-2 OS cells using AP1M2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92832_rabbit-polyclonal-ap1m2-antibody-4220251425186.jpg)
![Immunocytochemistry/ Immunofluorescence: AP1M2 Antibody - BSA Free [NBP2-92832] - Immunofluorescence analysis of L929 cells using AP1M2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92832_rabbit-polyclonal-ap1m2-antibody-42202514251849.jpg)
![Immunocytochemistry/ Immunofluorescence: AP1M2 Antibody - BSA Free [NBP2-92832] - Immunofluorescence analysis of H9c2 cells using AP1M2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92832_rabbit-polyclonal-ap1m2-antibody-4220251427528.jpg)
![Immunocytochemistry/ Immunofluorescence: AP1M2 Antibody - BSA Free [NBP2-92832] - Confocal immunofluorescence analysis of U2OS cells using AP1M2 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92832_rabbit-polyclonal-ap1m2-antibody-4220251429012.jpg)
![Western Blot: ART1 Antibody [NBP2-91995] - Analysis of extracts of various cell lines, using ART1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ART1-Antibody-Western-Blot-NBP2-91995-img0004.jpg "Western Blot: ART1 Antibody [NBP2-91995] - Analysis of extracts of various cell lines, using ART1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: ART1 Antibody [NBP2-91995] - Analysis of U-2 OS cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ART1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-91995-img0002.jpg)
![Immunohistochemistry-Paraffin: ART1 Antibody [NBP2-91995] - Paraffin-embedded human lung cancer using ART1 .](http://images.novusbio.com/fullsize/ART1-Antibody-Immunohistochemistry-Paraffin-NBP2-91995-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ART1 Antibody [NBP2-91995] - Analysis of L929 cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ART1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-91995-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0005.jpg "Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP2-87183] - Rabbit Anti-CHMP4B antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP2-87183-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0002.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Mouse. Target Name: CHMP4B. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0003.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target: CHMP4B. Positive control (+): MCF7 (N10). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0004.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0006.jpg)
![Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-278202415295525.jpg "Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32355) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-29820241435234.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-2920241192383.jpg)