| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11584 | |||
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." alt="LAG-3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human LAG-3 Monoclonal Antibody (NOVUS Catalog # NBP2-80059) at 1:150 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43793 | |||
| BSA Free | NBP2-46632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47452-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88966-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22537-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61710-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Immunofluorescence staining of Ramos cells using B7-2/CD86 antibody (SPM600).; followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SPM600-Immunocytochemistry-Immunofluorescence-NBP2-44515-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Immunofluorescence staining of Ramos cells using B7-2/CD86 antibody (SPM600).; followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.")
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Human Melanoma stained with CD86 Monoclonal Antibody (SPM600).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SPM600-Immunohistochemistry-Paraffin-NBP2-44515-img0001.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0014.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (2829) [NBP2-46632] - Staining of human cerebral cortex shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-Paraffin-NBP2-46632-img0003.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human cerebellum shows strong positivity in myelinated neural fiebrs.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0012.jpg)
![Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0009.jpg)
![Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0008.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0001.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse hippocampal formation shows strong immunoreactivity in myelinated fibers in the dentate gyrus.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0002.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat basal forebrain shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0004.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse cerebellum shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0013.jpg)
![Immunohistochemistry-Paraffin: FPR1 Antibody [NBP2-47452] - Staining of human tonsil shows weak cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/FPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-47452-img0005.jpg "Immunohistochemistry-Paraffin: FPR1 Antibody [NBP2-47452] - Staining of human tonsil shows weak cytoplasmic positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: FPR1 Antibody [NBP2-47452] - Staining of human colon tissue. Shows moderate cytoplasmic positivity in glandular cells and strong cytoplasmic positivity in peripheral leukocytes.](http://images.novusbio.com/fullsize/FPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-47452-img0002.jpg)
![Immunohistochemistry-Paraffin: FPR1 Antibody [NBP2-47452] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-47452-img0003.jpg)
![Immunohistochemistry-Paraffin: FPR1 Antibody [NBP2-47452] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/FPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-47452-img0004.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88966-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88966-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88966-img0017.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88966-img0014.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411124..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411220..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human cerebellum shows negative cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411310..jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cell line U-251 MG shows localization to endosomes and lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85741-img0014.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0019.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0016.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0017.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0018.jpg)
![Western Blot: CD2 Antibody (TS2/18) [NBP2-22537] - (Lane1), A-431 (Lane2), K562 (Lane 3) and Raji (Lane4). 1:4000 dilution.](http://images.novusbio.com/fullsize/CD2-Antibody-TS2-18-Western-Blot-NBP2-22537-img0008.jpg "Western Blot: CD2 Antibody (TS2/18) [NBP2-22537] - (Lane1), A-431 (Lane2), K562 (Lane 3) and Raji (Lane4). 1:4000 dilution.")
![Flow Cytometry: CD2 Antibody (TS2/18) [NBP2-22537] - Analysis of CD2 in PBMC cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD2-Antibody-TS2-18-Flow-Cytometry-NBP2-22537-img0005.jpg)
![Western Blot: CD2 Antibody (TS2/18) [NBP2-22537] - Analysis of 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates.](http://images.novusbio.com/fullsize/CD2-Antibody-TS2-18-Western-Blot-NBP2-22537-img0007.jpg)
![Flow Cytometry: CD2 Antibody (TS2/18) [NBP2-22537] - Analysis of CD2 in PBMC cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD2-Antibody-TS2-18-Flow-Cytometry-NBP2-22537-img0002.jpg)
![Flow Cytometry: CD2 Antibody (TS2/18) [NBP2-22537] - Analysis of CD2 in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD2-Antibody-TS2-18-Flow-Cytometry-NBP2-22537-img0004.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0004.jpg "Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against HEK293 (1) and ADRB2 (AA: 302-413)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis of MCF-7 cells using ADRB2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Flow-Cytometry-NBP2-61710-img0002.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mAb against human ADRB2 (AA: 302-413) recombinant protein. (Expected MW is 38.5 kDa)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0003.jpg)
![Western Blot: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Analysis using ADRB2 mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-Western-Blot-NBP2-61710-img0005.jpg)
![ELISA: beta-2 Adrenergic R/ADRB2 Antibody (5G3B5) [NBP2-61710] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/beta-2-Adrenergic-R-ADRB2-Antibody-5G3B5-ELISA-NBP2-61710-img0001.jpg)
![Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NB7588-img0006.jpg "Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7588-img0002.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0005.jpg)
![Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Flow-Cytometry-NB7588-img0004.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0008.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0007.jpg)
![Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0001.jpg)
