| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34992-1mg | |||
| Carrier-Free | NBP2-34992-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37388-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19249-0.1mg | |||
![Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Western-Blot-NBP2-37610-img0002.jpg "Western Blot: CREB Antibody (5G3) [NBP2-37610] - Analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody (5G3) [NBP2-37610] - Analysis of Hela cells using CREB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunocytochemistry-Immunofluorescence-NBP2-37610-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody (5G3) [NBP2-37610] - Analysis of prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-Immunohistochemistry-NBP2-37610-img0004.jpg)
![ELISA: CREB Antibody (5G3) [NBP2-37610] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CREB-Antibody-5G3-ELISA-NBP2-37610-img0001.jpg)
![SDS-Page: Human IFN-gamma Protein [NBP2-34992]](http://images.novusbio.com/fullsize/Human-IFN-gamma-Protein-SDS-Page-NBP2-34992-img0001.jpg "SDS-Page: Human IFN-gamma Protein [NBP2-34992]")
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0003.jpg "Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0007.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Flow-Cytometry-NBP2-37388-img0004.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0002.jpg)
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0005.jpg)
![ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-ELISA-NBP2-37388-img0001.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD74-Antibody-CerCLIP-1-Flow-Cytometry-NBP1-19249-img0001.jpg "Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-ECE-2-Immunohistochemistry-Frozen-NBP1-42312-img0001.jpg "Immunohistochemistry-Frozen: CCL2/MCP1 Antibody (ECE.2) [NBP1-42312] - Detection of CCL2/MCP1 in mouse testis. 10x dilution, 200x magnification.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Western-Blot-NBP2-67608-img0001.jpg "Western Blot: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human lung tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0005.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human tonsil tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human small intestine tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0003.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (SC68-07) [NBP2-67608] - Human prostate carcinoma tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-SC68-07-Immunohistochemistry-Paraffin-NBP2-67608-img0004.jpg)
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0002.jpg "Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.")
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0006.jpg "Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.")
![Flow Cytometry: CD10 Antibody (MEM-78) [NB500-372]-Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/p_444_1.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC-CyTM7.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0002.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-CyTM5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0003.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0004.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0005.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Western-Blot-NBP2-75441-img0006.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0005.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0003.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0007.jpg)