| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26878-25ul | |||
| Azide and BSA Free | NBP3-44281 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86935-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48330 | |||
| Azide and BSA Free | NBP3-11580 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44338 | |||
| Azide and BSA Free | NBP2-34732-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18550-25ul | |||
| Azide and BSA Free | NBP3-44371 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38459-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26878-100ul | |||
| BSA Free | NBP3-26878-25ul | |||
![Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Whole cell lysate (10 ug) from MOLT-4, HEK293T, U2OS, Jurkat, A-549, GaMG, MCF-7, and HUVEC-C cells prepared using NETN lysis buffer. Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-BLR127H-Western-Blot-NBP3-14710-img0003.jpg "Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Whole cell lysate (10 ug) from MOLT-4, HEK293T, U2OS, Jurkat, A-549, GaMG, MCF-7, and HUVEC-C cells prepared using NETN lysis buffer. Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Rabbit anti- CD31/PECAM-1 recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-BLR127H-Immunohistochemistry-NBP3-14710-img0002.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] -: Rabbit antiCD31/PECAM-1 recombinant monoclonal antibody[BLR127H]. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14710_rabbit-cd31-pecam-1-mab-immunohistochemistry-paraffin-122202412441..jpg)
![Rabbit anti-CD31 recombinant monoclonal [BLR127H] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp3-14710_rabbit-cd31-pecam-1-mab-flow-cytometry-2372025115630..png)
![Western Blot: CD168 Antibody [NBP2-15781] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CD168-Antibody-Western-Blot-NBP2-15781-img0001.jpg "Western Blot: CD168 Antibody [NBP2-15781] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-15781] - RHAMM/CD168 Antibody detects RHAMM/CD168 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RHAMM/CD168 Antibody dilution: 1:250. Scale bar = 100 um.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-15781-img0005.jpg)
![Immunohistochemistry-Paraffin: CD168 Antibody [NBP2-15781] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-15781-img0002.jpg)
![Immunoprecipitation: RHAMM/CD168 Antibody [NBP2-15781] - Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 ug of RHAMM antibody [N1N2], N-term. Western blot analysis was performed using RHAMM antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunoprecipitation-NBP2-15781-img0004.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Western-Blot-NBP2-75441-img0006.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0005.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0003.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0007.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Western-Blot-NBP2-44340-img0005.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.10).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Tonsil stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0003.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0004.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Immunohistochemistry-Paraffin-NBP2-44340-img0002.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.10) [NBP2-44340] - Human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-10-Flow-Cytometry-NBP2-44340-img0001.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (SPM122).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM122-Immunohistochemistry-Paraffin-NBP2-32930-img0001.jpg "Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (SPM122).")
![Flow Cytometry: CD31/PECAM-1 Antibody (SPM122) [NBP2-32930] - Flow Cytometric analysis of human CD31/PECAM-1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled CD31/PECAM-1 Monoclonal Antibody (SPM122).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM122-Flow-Cytometry-NBP2-32930-img0002.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0006.jpg "Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human ovarian cancer (mucinous carcinoma) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0001.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human ovarian cancer (yolk sack tumor) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0002.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0003.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human glioma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0004.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human pancreas shows strong membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0005.jpg)
![Immunohistochemistry-Paraffin: CD99 Antibody (CL12929) [NBP3-18550] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD99-Antibody-CL12929-Immunohistochemistry-Paraffin-NBP3-18550-img0007.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Analysis in human testis and prostate tissues using NBP2-38459 antibody. Corresponding HMMR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Analysis in human testis and prostate tissues using NBP2-38459 antibody. Corresponding HMMR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP2-38459] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP2-38459-img0002.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0010.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human testis shows strong cytoplasmic/ membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0008.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human tonsil shows strong cytoplasmic/ membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0009.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0011.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Western-Blot-NBP2-27551-img0002.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Tonsil (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0006.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of human skin Epidermis shows detection on the apical side of the basal cell layer.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Testis (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0005.jpg)
![Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-278202415212458.jpg "Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-29820241411547.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunocytochemistry analysis of HeLa cells labeling RHAMM/CD168 with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-2920241114816.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Flow cytometric analysis of HeLa cells labeling RHAMM/CD168. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32129, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-392024884629.jpg)