| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0470A488-100 | |||
| Alexa Fluor 546 | DDX0470A546-100 | |||
| Alexa Fluor 647 | DDX0470A647-100 | |||
| Biotin | DDX0470B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27092 | |||
| BSA Free | NBP2-27091SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25241SS | |||
| Azide and BSA Free | NBP2-80965 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76465-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76739-0.025mg | |||
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0002.jpg "Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]")
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0001.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Sections of nasal biopsies were incubated with antibodies against TLR9 (C) visualized by 3, 3-diaminobenzidine (brown). In control slides (F), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0098239) licensed under a CC-BY license. TLR7 Antibody [NB100-56682]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0003.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz. TLR7 Antibody [NBP2-24767]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0004.jpg)
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary. TLR7 Antibody [NB100-56588]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0005.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Western-Blot-DDX0470P-100-img0007.jpg "Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06")
![Immunohistochemistry-Frozen: TLR3 Antibody (619F7.06) [DDX0470P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0470P-100. Staining of human tonsil section.](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Frozen-DDX0470P-100-img0003.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of canine blood cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (619F7.06) [DDX0470P-100] - Paraffin staining of Canine keratinocytes with TLR3 Antibody](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Paraffin-DDX0470P-100-img0001.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human breast carcinoma CAMA-1 cellline with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0004.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human monocyte-derived DCs and human EBV B cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0005.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Western Blot: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis using IL-23 receptor antibody. Lysate from (A) human HCT116, (B) Jurkat and C) mouse NIH 3T3 cells probed with IL-23 receptor antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Western-Blot-NBP2-27091-img0004.jpg "Western Blot: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis using IL-23 receptor antibody. Lysate from (A) human HCT116, (B) Jurkat and C) mouse NIH 3T3 cells probed with IL-23 receptor antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: IL-23 R Antibody (15N6C6) [NBP2-27091] - Human Appendix FFPE tissue section stained with IL-23 R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Immunohistochemistry-Paraffin-NBP2-27091-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-23 R Antibody (15N6C6) [NBP2-27091] - Analysis of IL-23 receptor in human colon tissue using IL-23 receptor antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IL-23-R-Antibody-15N6C6-Immunohistochemistry-Paraffin-NBP2-27091-img0003.jpg)
![Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Western-Blot-NBP2-25241-img0001.jpg "Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.")
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STAT6-Antibody-177C322-1-Immunocytochemistry-Immunofluorescence-NBP2-25241-img0003.jpg)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NB100-56363-img0003.jpg "Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0006.jpg)
![Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunohistochemistry-Paraffin-NB100-56363-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56363-img0005.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0005.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (A103+M2-7C10+M2-9E3). Note cytoplasmic staining of cells.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Paraffin-embedded human melanoma. pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0002.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) - Azide and BSA Free [NBP2-34546] - Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103 + M2-7C10 + M2-9E3) [NBP2-34244]](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-+-M2-7C10-+-M2-9E3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34546-img0004.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202419463463.gif "<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Western Blot Validation of MyD88 in human cell lines. Loading: 15 ug of lysates per lane. Antibodies: NBP1-76465 (2 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0010.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - Animal Species ReactivityLoading: Lysates/proteins at 15 ug per lane.Antibodies: NBP1-76925 (2 ug/mL) or NBP1-76465 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MyD88 Antibody - BSA Free [NBP1-76465] - Validation of MyD88 In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202420392736.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunocytochemistry-immunofluorescence-22102025124527.gif)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025124757.jpg)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Mouse Heart. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025125425.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0011.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.")
![Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunohistochemistry-Frozen-NB200-610-img0018.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0007.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0001.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0008.jpg)
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0015.jpg)
![Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-NBP1-76739-img0006.jpg "Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)). White bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76739-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in Western Blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0008.jpg)
![Western Blot: IL-21R Antibody [NBP1-76739] - Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 ug /ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Western-Blot-NBP1-76739-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-21R Antibody [NBP1-76739] - Rat lung with IL-21 receptor antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IL-21R-Antibody-Immunohistochemistry-Paraffin-NBP1-76739-img0001.jpg)
![Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - Treatment of avian xenografts with IL-21 upregulates Blimp-1 expression. (A) The three-dimensional growth of BxPC-3 cells transplanted on the CAM of fertilized chicken eggs & resected tumors (three replicates) from vehicle control & IL-21 were photographed at day 17 of embryonic development. Upper row for vehicle control; lower row for IL-21-treated (150 ng/mL) tumors. (B) HE & IL-21R (brown) IHC staining of vehicle control & IL-21-treated (150 ng/mL) resected xenograft tumors. (C) Comparison & quantification of Blimp-1 (nuclei: red) expression of vehicle control & IL-21-treated (150 ng/mL) xenograft tumors. **** P Green: IL-21 Receptor Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-271120242011556.gif)
![Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76739_rabbit-polyclonal-il-21r-antibody-2711202419241320.jpg)
