| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28142 | |||
| FITC | NBP1-28141 | |||
| PE | NBP1-28143 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35252-1mg | |||
| Carrier-Free | NBP2-35252-100ug | |||
| Carrier-Free | NBP2-35252-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56439SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44737 | |||
| Azide and BSA Free | NBP2-34791-0.1mg | |||
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the PE conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0004.jpg "Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the PE conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.")
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - 1 ug/106 BALB/c splenocytes were incubated with either hamster IgG (as an isotype control) or purified hamster anti-mouse CD79, and then stained with mouse anti-hamster IgG (H+L)-FITC. Small lymphocytes were gated and analyzed on a flow cytometer](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0001.jpg)
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the Biotin conjugate of NBP1-28140. Multiple staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0002.jpg)
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the FITC conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0003.jpg)
![Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Western-Blot-NBP1-42561-img0011.jpg "Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunocytochemistry-Immunofluorescence-NBP1-42561-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunohistochemistry-Paraffin-NBP1-42561-img0010.jpg)
![Flow Cytometry: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Flow-Cytometry-NBP1-42561-img0012.jpg)
![SDS-Page: Rat IL-4 Protein [NBP2-35252]](http://images.novusbio.com/fullsize/Rat-IL-4-Protein-SDS-Page-NBP2-35252-img0001.jpg "SDS-Page: Rat IL-4 Protein [NBP2-35252]")
![Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.](http://images.novusbio.com/fullsize/Recombinant-Rat-IL-4-Protein-Bioactivity-NBP2-35252-img0002.jpg)
![Western Blot: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IgJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IgJ.](http://images.novusbio.com/fullsize/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Western-Blot-NBP2-71016-img0001.jpg "Western Blot: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IgJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IgJ.")
![Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Staining of paraffin-embedded Human lymphoma tissue using anti-IgJ mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71016-img0004.jpg)
![Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Analysis of Jurkat cells, using anti-IgJ antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71016-img0003.jpg)
![Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Staining of paraffin-embedded Human tonsil using anti-IgJ mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71016-img0005.jpg)
![Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Analysis of Hela cells, using anti-IgJ antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71016-img0002.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Knockout-Validated-NBP1-03250-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP1-03250-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunohistochemistry-Paraffin-NBP1-03250-img0001.jpg)
![Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-288202412461675.jpg "Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29820241492635.jpg)
![Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-292024119237.jpg)
![Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29202416453215.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP3-09107-img0001.jpg "Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0002.jpg)
![Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-278202415295561.jpg "Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD21 Antibody (PD00-23) [NBP3-32135] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD21 antibody (NBP3-32135) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32135) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-298202413585521.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody (PD00-23) [NBP3-32135] - Immunocytochemistry analysis of Raji cells labeling CD21 with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202410484856.jpg)
![Flow Cytometry: CD21 Antibody (PD00-23) [NBP3-32135] - Flow cytometric analysis of Raji cells labeling CD21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32135, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202416453281.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-278202415242671.jpg "Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29820241492652.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunocytochemistry analysis of SiHa cells labeling IL-7R alpha/CD127 with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29202410484826.jpg)
![Flow Cytometry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Flow cytometric analysis of K-562 cells labeling IL-7R alpha/CD127. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32122, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-392024884614.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: IL-21 R Antibody [NB100-56439] - Analysis of IL-21 R in 30 ug of Raji cell lysate using this antibody.](http://images.novusbio.com/fullsize/IL-21-R-Antibody-Western-Blot-NB100-56439-img0003.jpg "Western Blot: IL-21 R Antibody [NB100-56439] - Analysis of IL-21 R in 30 ug of Raji cell lysate using this antibody.")
![Western Blot: CD79A Antibody (SPM549) [NBP2-32833] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (SPM549).](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM549-Western-Blot-NBP2-32833-img0004.jpg "Western Blot: CD79A Antibody (SPM549) [NBP2-32833] - Western Blot Analysis of human Raji cell lysate using CD79A Antibody (SPM549).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (SPM549) [NBP2-32833] - Immunofluorescence Analysis of PFA-fixed Raji cells labeling CD79a with CD79A Antibody (SPM549) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM549-Immunocytochemistry-Immunofluorescence-NBP2-32833-img0002.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (SPM549) [NBP2-32833] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (SPM549)](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM549-Immunohistochemistry-Paraffin-NBP2-32833-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (SPM549) [NBP2-32833] - Formalin-fixed paraffin-embedded human tonsil stained with CD79a Monoclonal antibody (SPM549)](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM549-Immunohistochemistry-Paraffin-NBP2-32833-img0001.jpg)