| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40415SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24552SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25259 | |||
| Azide and BSA Free | NBP2-25259SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43956 | |||
| BSA Free | NBP2-34488-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86166-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39004-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82627-25ul | |||
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Western Blot: Nurr1 Antibody [NB110-40415] - Western blot detection of Nurr1 in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nurr1 polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3.](http://images.novusbio.com/fullsize/p_40415_1.jpg "Western Blot: Nurr1 Antibody [NB110-40415] - Western blot detection of Nurr1 in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nurr1 polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3.")
![Immunohistochemistry: Nurr1 Antibody [NB110-40415] - Nurr1 staining with Nurr1 polyclonal antibody at a 1:100 dilution in adult rat brain hippocampus. Tissue was fixed in paraformaldehyde and cut into 20 micron sections.](http://images.novusbio.com/fullsize/Nurr1-Antibody-Immunohistochemistry-NB110-40415-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-24552-img0001.jpg "Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.")
![Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552] - IHC analysis of human spleen using NBP2-24552 at 10 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP2-24552-img0002.jpg)
![Simple Western: CD97 Antibody [NBP2-24552] - Simple Western lane view shows a specific band for CD97 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD97-Antibody-Simple-Western-NBP2-24552-img0003.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0004.jpg "Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.")
![Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [NBP2-25245] - FFPE human esophageal squamous cell carcinoma stained with TLR2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Immunohistochemistry-Paraffin-NBP2-25245-img0003.jpg)
![Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cell-Surface-NBP2-25245-img0005.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of (A) TLR2 partial recombinant protein, (B) transfected cell lysate, and (C) control 293T lysate using TLR2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0001.jpg)
![Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245] - (A) Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. (B) Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cytometry-NBP2-25245-img0002.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining in human tonsil and cerebral cortex tissues. Corresponding CD40 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining in human tonsil and cerebral cortex tissues. Corresponding CD40 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Lane 1: Marker [kDa] Lane 2: Human spleen](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Western-Blot-NBP2-34488-img0014.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Human stomach cancer FFPE block stained with CD40 antibody at 1:6000 overnight. Antigen retrieval at pH 9. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0020.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0016.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human colon shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0017.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-CL1673-Immunohistochemistry-Paraffin-NBP2-34488-img0018.jpg)
![Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-BL-178-12C7-Western-Blot-NBP3-14668-img0013.jpg "Western Blot: CD45 Antibody (BL-178-12C7) [NBP3-14668] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 1 second. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody [BL-178-12C7](A700-012). Secondary: HRP-conjugated goat anti-rabbitIgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0006.jpg)
![Immunohistochemistry: CD45 Antibody [NBP3-14668] - Sample: FFPE section of human coloncarcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™.Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0007.jpg)
![Flow Cytometry: CD45 Antibody [NBP3-14668] - Sample: Jurkat cells were fixed in 4% formaldehyde andpermeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45recombinant monoclonal antibody (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP3-14668-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (BL-178-12C7) [NBP3-14668] - CD45 Antibody [NBP3-14668] - Antibody: Rabbit anti-CD45recombinant monoclonal antibody. Secondary: HRP-conjugated goat antirabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunohistochemistry-NBP3-14668-img0004.jpg)
![Immunoprecipitation: CD45 Antibody [NBP3-14668] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from Jurkat cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-CD45recombinant monoclonal antibody used for IP at 20 ul/mg lysate. CD45 wasalso immunoprecipitated by a previous lot of this antibodyand rabbit anti-CD45 antibody. For blotting immunoprecipitated CD45,was used at 1:1000. Chemiluminescence withan exposure time of 250 milliseconds.](http://images.novusbio.com/fullsize/CD45-Antibody-Immunoprecipitation-NBP3-14668-img0002.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0018.jpg "Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86166 (A) shows similar protein distribution across tissues to independent antibody NBP1-86168 (B).](http://images.novusbio.com/fullsize/CD14-Antibody-Western-Blot-NBP1-86166-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0014.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0015.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0016.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0017.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD14-Antibody-Immunohistochemistry-Paraffin-NBP1-86166-img0019.jpg)
![Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining in human lung and pancreas tissues . Corresponding MARCO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-Paraffin-NBP2-39004-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining in human lung and pancreas tissues . Corresponding MARCO RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: MARCO Antibody [NBP2-39004] - Staining in normal human liver. Image from a verified customer review.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-NBP2-39004-img0014.jpg)
![Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining of human liver shows strong positivity in Kupffer cells.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-Paraffin-NBP2-39004-img0009.jpg)
![Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining of human lung shows strong positivity in macrophages.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-Paraffin-NBP2-39004-img0010.jpg)
![Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining of human lymph node shows moderate membranous positivity.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-Paraffin-NBP2-39004-img0011.jpg)
![Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/MARCO-Antibody-Immunohistochemistry-Paraffin-NBP2-39004-img0012.jpg)
![Western Blot: DNAJC2 Antibody [NBP1-82627] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Western-Blot-NBP1-82627-img0007.jpg "Western Blot: DNAJC2 Antibody [NBP1-82627] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: DNAJC2 Antibody [NBP1-82627] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82627-img0009.jpg)
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP1-82627] - Staining of human prostate shows weak cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP1-82627-img0014.jpg)
![Western Blot: DNAJC2 Antibody [NBP1-82627] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Western-Blot-NBP1-82627-img0006.jpg)
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP1-82627] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP1-82627-img0012.jpg)
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP1-82627] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP1-82627-img0013.jpg)