| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72631 | |||
| Biotin | NBP1-73508 | |||
| FITC | NBP1-73280 | |||
| HRP | NBP1-73572 | |||
| Rhodamine | NBP1-73278 | |||
| Texas Red | NBP1-73509 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7581 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72627 | |||
| Biotin | NBP1-73437 | |||
| FITC | NB120-6810 | |||
| HRP | NBP1-73541 | |||
| Rhodamine | NB7576 | |||
| Texas Red | NB7598 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NB120-6823 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB120-6578 | |||
| FITC | NB120-6521-1000ug | |||
| HRP | NB120-6667 | |||
| Rhodamine | NB120-6522 | |||
| Texas Red | NB120-6523 | |||
![Western Blot: Rabbit anti-Chicken IgG F(ab)2 Secondary Antibody [Unconjugated] [NBP1-72800] - Lane 1: Chicken IgG whole molecule (NBP1-97037). Lane 2: Chicken IgG F(c) Fragment (NBP1-96786). Lane 3: Chicken IgG F(ab) Fragment (NBP1-96773). All samples were reduced. Load: 50 ng per lane; incubated with blocking buffer for 30 min at RT. Primary Antibody: NBP1-72800 incubated for 60 min at RT. Secondary antibody: Goat anti-Rabbit IgG, peroxidase Conjugated antibody incubated in blocking buffer for 30 min at RT. Predicted/observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-F-ab-2-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72800-img0003.jpg "Western Blot: Rabbit anti-Chicken IgG F(ab)2 Secondary Antibody [Unconjugated] [NBP1-72800] - Lane 1: Chicken IgG whole molecule (NBP1-97037). Lane 2: Chicken IgG F(c) Fragment (NBP1-96786). Lane 3: Chicken IgG F(ab) Fragment (NBP1-96773). All samples were reduced. Load: 50 ng per lane; incubated with blocking buffer for 30 min at RT. Primary Antibody: NBP1-72800 incubated for 60 min at RT. Secondary antibody: Goat anti-Rabbit IgG, peroxidase Conjugated antibody incubated in blocking buffer for 30 min at RT. Predicted/observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [NBP1-72800] - Chicken IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-F-ab-2-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72800-img0004.jpg)
![Western Blot: Rabbit anti-Porcine IgG (H+L) Secondary Antibody [NBP1-73812] - Lane 1: Swine IgG Load: 100 ng per lane Primary antibody: Porcine IgG (H+L) Antibody incubated overnight at 4C. Secondary antibody: Rabbit anti-Porcine IgG (H+L) Secondary Antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.](http://images.novusbio.com/fullsize/Rabbit-anti-Porcine-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-73812-img0002.jpg "Western Blot: Rabbit anti-Porcine IgG (H+L) Secondary Antibody [NBP1-73812] - Lane 1: Swine IgG Load: 100 ng per lane Primary antibody: Porcine IgG (H+L) Antibody incubated overnight at 4C. Secondary antibody: Rabbit anti-Porcine IgG (H+L) Secondary Antibody incubated for 30 min at RT; incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.")
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-61220242151820.jpg "Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Prostate cancer Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202421351278.jpg)
![Western Blot: Orosomucoid 2 Antibody (D7) [NBP3-41442] - Sample: Lane1: Human Serum; Lane2: Rat Liver lysate; Lane3: Rat Pancreas lysate; Lane4: Rat Bladder lysate Primary Ab: 3ug/ml NBP3-41442; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202420484723.jpg)
![Western Blot: Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-62589] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/Donkey-F-ab-2-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-62589-img0001.jpg "Western Blot: Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-62589] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Image from the PE version of this antibody.")
![ELISA: Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-62589] - ELISA results of purified Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Rabbit IgG. Each well was coated in duplicate with 1.0 ug of Rabbit IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer](http://images.novusbio.com/fullsize/Donkey-F-ab-2-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-62589-img0002.jpg)
![Western Blot: Chicken anti-Mouse IgG (H+L) Secondary Antibody [NB7593] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein chicken secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB7593-img0001.jpg "Western Blot: Chicken anti-Mouse IgG (H+L) Secondary Antibody [NB7593] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein chicken secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image using the FITC form of this antibody.")
![ELISA: Chicken anti-Mouse IgG (H+L) Secondary Antibody [NB7593] - ELISA results of purified Chicken anti-Mouse IgG (H+L) Secondary Antibody [Biotin] tested against Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG (blue line).The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Streptavidin HRP conjugate, and TMB substrate.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB7593-img0003.jpg)
![Western Blot: Goat anti-Human IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72710] - Analysis using recombinant human IgG1 lysate. From a verified customer review. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72710-img0003.jpg "Western Blot: Goat anti-Human IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72710] - Analysis using recombinant human IgG1 lysate. From a verified customer review. Image using the HRP format of this antibody.")
![ELISA: Goat anti-Human IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72710] - ELISA results of purified Goat anti-Human IgG Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 ug of Human IgG. The starting dilution of antibody was 5 ug/ml and the X-axis represents the log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as a blocking buffer and TMB substrate. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-H+L-Secondary-Antibody-Unconjugated-ELISA-NBP1-72710-img0002.jpg)
![Dot Blot: Goat anti-Human IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72710] - Lane 1: 100 ng. Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Secondary Antibody: NB120-6858, incubated with blocking buffer for 1 hour at RT. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-H+L-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72710-img0001.jpg)
![ELISA: Goat anti-Canine IgG Fc Secondary Antibody [NBP1-72772] - ELISA Results of Purified Goat anti-Canine IgG Fc Secondary antibody tested against purified Dog IgG Fc. Each well was coated in duplicate with 10 ug of Dog IgG Fc [Red Line] and Dog IgG [Green Line]. The working dilution of Dog IgG Fc is 1:48,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Donkey Anti-Goat IgG mx8 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgG-Fc-Secondary-Antibody-ELISA-NBP1-72772-img0001.jpg "ELISA: Goat anti-Canine IgG Fc Secondary Antibody [NBP1-72772] - ELISA Results of Purified Goat anti-Canine IgG Fc Secondary antibody tested against purified Dog IgG Fc. Each well was coated in duplicate with 10 ug of Dog IgG Fc [Red Line] and Dog IgG [Green Line]. The working dilution of Dog IgG Fc is 1:48,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Donkey Anti-Goat IgG mx8 HRP conjugated and TMB substrate.")
![Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Observed/Predicted size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NBP2-62585-img0001.jpg "Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Observed/Predicted size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image from the HRP version of this antibody.")
![Dot Blot Results of Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab')2 Goat Anti-Mouse IgG at 1.0ug/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1ug/mL for 30 mins at RT. Block: BlockOut Buffer at RT for 30 mins. Exposure: 1 sec.](http://images.novusbio.com/fullsize/nbp2-62585_goat-polyclonal-goat-f-ab-2-anti-mouse-igg-h-l-secondary-antibody-unconjugated-23520231752222.jpg)
![Dot Blot Results of F(ab')2 Goat Anti-Mouse IgG Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab')2 Goat Anti-Mouse IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1µg/mL for 30 mins at RT. Block: BlockOut Buffer [p/n MB-073] at RT for 30 mins. Exposure: 1 sec.](http://images.novusbio.com/fullsize/nbp2-62585_goat-polyclonal-goat-f-ab-2-anti-mouse-igg-h-l-secondary-antibody-unconjugated-187202581871.jpg)
![Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72743] - Western Blot of Donkey anti-Rat IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Donkey anti-Rat IgG (H+L) Secondary antibody (Pre-adsorbed) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Donkey-anti-Rat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72743-img0006.jpg "Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72743] - Western Blot of Donkey anti-Rat IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Donkey anti-Rat IgG (H+L) Secondary antibody (Pre-adsorbed) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgM Isotype Control-Western Blot-NBP1-96968-img0003.jpg "Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize/Rabbit-IgM-Isotype-Control-SDS-Page-NBP1-96968-img0001.jpg)
![Western Blot: KIR2DS4/CD158i Antibody (C3) [NBP3-41615] - Sample: Rat Lymph node lysate; Primary Ab: 3ug/ml NBP3-41615; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41615_mouse-kir2ds4-cd158i-mab-c3-61220242055468.jpg "Western Blot: KIR2DS4/CD158i Antibody (C3) [NBP3-41615] - Sample: Rat Lymph node lysate; Primary Ab: 3ug/ml NBP3-41615; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: KIR2DS4/CD158i Antibody (C3) [NBP3-41615] - Sample: Rat Lymph node lysate; Primary Ab: 3ug/ml NBP3-41615; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41615_mouse-kir2ds4-cd158i-mab-c3-61220242112136.jpg)
![Immunohistochemistry-Paraffin: KIR2DS4/CD158i Antibody (C3) [NBP3-41615] - DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20ug/ml NBP3-41615; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41615_mouse-kir2ds4-cd158i-mab-c3-61220242055490.jpg)
![Western Blot: Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NB120-6520] - ELISA results of purified Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [Biotin] tested against purified Goat IgG. Each well was coated in duplicate with 1.0 ug of Goat IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Donkey-F-ab-anti-Goat-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NB120-6520-img0002.jpg "Western Blot: Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NB120-6520] - ELISA results of purified Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [Biotin] tested against purified Goat IgG. Each well was coated in duplicate with 1.0 ug of Goat IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer. Using the Biotin format of this antibody.")
![Western Blot: Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] [NB120-6520] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Donkey-F-ab-anti-Goat-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NB120-6520-img0001.jpg)