![ELISA: Goat anti-Canine IgG Fc Secondary Antibody [HRP] [NBP1-73587] - ELISA Results of Goat anti-Canine IgG Fc Secondary antibody [HRP] tested against purified Dog IgG Fc HRP. Each well was coated in duplicate with 1.0 ug of Dog IgG Fc. The working dilution is 1:46,500. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgG-Fc-Secondary-Antibody-[HRP]-ELISA-NBP1-73587-img0001.jpg "ELISA: Goat anti-Canine IgG Fc Secondary Antibody [HRP] [NBP1-73587] - ELISA Results of Goat anti-Canine IgG Fc Secondary antibody [HRP] tested against purified Dog IgG Fc HRP. Each well was coated in duplicate with 1.0 ug of Dog IgG Fc. The working dilution is 1:46,500. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.")
| Reactivity | CaSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | HRP |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Dog IgG, Dog IgG F(c) and Dog Serum |
| Immunogen | Dog IgG F(c) fragment |
| Specificity | No reaction was observed against Dog IgG F(ab). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by ELISA. This secondary antibody anti-Dog is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:40,000 of the reconstitution concentration is suggested for this product. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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