Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody [HRP] [NBP1-73749] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat ...read more
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Immunogen affinity purified
Western Blot 1:1000 - 1:10000
ELISA 1:10000 - 1:50000
Immunohistochemistry 1:500 - 1:2500
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:15,000 - 1:60,000 is suggested for this product.
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