Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody

Western Blot: Rabbit anti-Chicken IgG F(ab)2 Secondary Antibody [Unconjugated] [NBP1-72800] - Lane 1: Chicken IgG whole molecule (NBP1-97037). Lane 2: Chicken IgG F(c) Fragment (NBP1-96786). Lane 3: Chicken IgG F(ab) Fragment (NBP1-96773). All samples were reduced. Load: 50 ng per lane; incubated with blocking buffer for 30 min at RT. Primary Antibody: NBP1-72800 incubated for 60 min at RT. Secondary antibody: Goat anti-Rabbit IgG, peroxidase Conjugated antibody incubated in blocking buffer for 30 min at RT. Predicted/observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.

Dot Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [NBP1-72800] - Chicken IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.

Western Blot of Unconjugated Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody

• Reactivity: Ch
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Chicken IgG F(ab')2 fragment
• Specificity: No reaction was observed against Chicken IgG F(c).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:2000 - 1:10000
• Application Notes: This product has been tested by ELISA and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.