Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] (Pre-absorbed)

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Western Blot: Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] [NB7581] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
PE
Format
Pre-absorbed
Concentration
LYOPH

Order Details

Donkey F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] (Pre-absorbed) Summary

Immunogen
Rabbit IgG whole molecule
Localization
Secreted
Specificity
Rabbit IgG (H & L)
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 10000
  • Flow Cytometry 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required - stain 10^6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Reactivity Notes

This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute to desired concentration.

Background

Can be used in immunomicroscopy and flow cytometry or analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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